钙稳态失衡在氯化镧诱导MDCC-MSB1细胞凋亡中的作用  

Effect of the Disequilibrium of Calcium Homeostasis on the Apoptosis of MDCC-MSB1 Cells Induced by Lanthanum Chloride

作　　者：赵兰[1] 李文军[1] 王金涛[1] 徐世文[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《中国家禽》2008年第3期9-12,共4页China Poultry

摘　　要：为研究钙稳态失衡在LaCl3诱导MDCC-MSB1细胞凋亡中的作用,将MDCC-MSB1细胞常规培养于RPMI-1640培养液中,加入终浓度为0.5、1.0、1.5、2.0、2.5、3.0、3.5和4.0mmol/L的LaCl3,继续培养24h后,应用MTT法检测细胞增殖抑制率,DNALadder法和TUNEL法检测细胞凋亡,以Fura-2为荧光探针检测细胞内[Ca2+]i的变化。在LaCl3浓度为0.5～4.0mmol/L范围内,细胞的增殖抑制率增加,细胞凋亡数量和细胞内[Ca2+]i呈升高趋势,并呈剂量-效应关系。结果表明,LaCl3能抑制MDCC-MSB1细胞的增殖,并可能通过改变[Ca2+]i而诱导其发生凋亡。To study the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride,MDCC-MSB1 cells was conventionally cultured in RPMI-1640 for 24 h,which was added with concentration are 0.5,1.0,1.5,2.0,2.5,3.0,3.5 and 4.0 mmol/L, to utilize MTT to detect the cell multiplication inhibition ratio,use DNA Ladder and TUNEL to detect apoptosis,use the Fura-2/AM as the probe to detect the calcium ion within nerve cells concentration （[Ca^2＋ ]i）. LaCl3 concentration is in the confine of 0.5 to 4.0 mmol/L,cell multiplication inhibition ratio was increasing,apoptosis and [Ca^2＋]i were offering an increasing tendency,which present dose- effectiveness relationship. The results showed that LaCl3 could restrain the proliferation of MDCC-MSB1 cells and derive apoptosis happens by changing[Ca^2＋]i.

关 键 词：氯化镧 MDCC—MSB1 DNA损伤 钙稳态 凋亡 

分 类 号：Q255[生物学—细胞生物学] X132[环境科学与工程—环境科学]