免疫抑制剂对HaCaT细胞分泌CXCL11/I-TAC的影响  

Effect of Immunosuppressants on Secretion of CXCL11/I-TAC by HaCaT Cells

作　　者：肖汀[1] 郭英军[1] 吴江[1] 吴剑[1] 王雅坤[1] 何春涤[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科,沈阳110001

出　　处：《中国医科大学学报》2008年第1期16-18,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30400389)

摘　　要：目的观察免疫抑制剂对人角质形成细胞系HaCaT分泌CXCL11/I-TAC的影响。方法用逆转录-多聚酶链反应(RT-PCR)检测HaCaT细胞中CXCL11/I-TAC的表达。用酶联免疫吸附实验(ELISA)检测不同浓度的甲氨蝶呤、地塞米松和雷公藤内酯醇作用后培养24h的HaCaT细胞上清中的CXCL11/I-TAC水平。结果无刺激条件下HaCaT细胞本身不表达CXCL11/I-TAC mRNA。IFN-γ和TNF-α均可诱导HaCaT细胞表达CXCL11/I-TAC mRNA。本研究首次报告,甲氨蝶呤(0.1,1,10ng/ml)、地塞米松(0.01,0.1,1,10ng/ml)和雷公藤内酯醇(0.01,0.1,1ng/ml)均显著抑制IFN-γ和TNF-α诱导的HaCaT细胞分泌的CXCL11/I-TAC水平,差异有统计学意义;并且抑制作用均呈明显的剂量依赖性。结论甲氨蝶呤、地塞米松和雷公藤内酯醇对Th1占主导的炎性皮肤病的治疗机制可能均与其抑制角质形成细胞分泌CXCL11/I-TAC有关,从而减少Th1细胞进入皮肤,减轻炎性反应。Objective To investigate the effect of immunosuppressants on CXCL11/I-TAC secretion by a human keratinocyte cell line,HaCaT cells. Methods Reverse transcription-PCR （RT-PCR） was performed to detect the CXCL11/I-TAC expression in HaCaT cells. Enzyme-linked immunosorbent assay （ELISA） was conducted to detect the supematant CXCL11/I-TAC levels of 24 h cultured HaCaT cells af- ter exposure to various concentrations of methotrexate,dexamethasone and triptolide. Results HaCaT cells did not express CXCL11/I-TAC mRNA without stimulation. Upon stimulation of IFN-γ/or TNF-oL,the CXCL11/I-TAC mRNA expression was induced in HaCaT cells. It was reported for the first time that methotrexate（0.1,1,10 ng/ml）,dexamethasone （0.01,0.1,1,10 ng/ml） and triptolide （0.01,0.1,1 ng/ml） inhibited the CXCL11/I-TAC secretion induced by the IFN-γand TNF-α in HaCaT cells,with statistical significance. Moreover,the inhibitory effect exhibited obvious dose-dependency. Conclusion The therapeutic mechanisms of methotrexate,dexamethasone and triptolide for Th1-dominated skin diseases may be associated with their inhibitory effects on CXCL11/I-TAC secretion from kemtinocytes, consequently inhibiting the Thl cells infiltration and the subsequent Th1 amplification circuit of inflammation in the skin.

关 键 词：CXCL11/I—TAC HACAT细胞 甲氨蝶呤 地塞米松 雷公藤内酯醇 

分 类 号：R735.7[医药卫生—肿瘤]