高效液相色谱法测定粮食中伏马菌素B1  

Detection of Fumonisin B_1 in Samples of Maize by High Performance Liquid Chromatography

作　　者：宫慧芝[1] 计融[2] 李岩溪[3] 谢妮[1] 杨军[1] 

机构地区：[1]中国医科大学公共卫生学院卫生检验教研室,沈阳110001 [2]中国疾病预防控制中心营养与食品安全所,北京100050 [3]中国医科大学90期7年制,沈阳110001

出　　处：《中国医科大学学报》2008年第1期59-61,共3页Journal of China Medical University

基　　金：“十五”国家科技重大专项项目(2001BA804A3205)

摘　　要：目的建立伏马菌素B(1FB1)的高效液相色谱检测方法;了解辽宁地区玉米产品中FB1的污染状况。方法利用强离子交换固相提取柱(SPE柱)净化样品提取液,选用C18反相柱,以甲醇∶NaH2PO4为流动相,流速1ml/min;荧光检测器检测。结果1μg/ml、5μg/ml、10μg/ml3个加标浓度的回收率在71.77%～102.68%之间,平均回收率为84.72%,总变异系数为12.17%。检测78份玉米样品,阳性率为32.05%,阳性样品中FB1平均含量为(0.74±0.05)μg/g。结论建立了用高效液相色谱法检测FB1的方法;本实验检测结果提示辽宁地区玉米样品的FB1污染率较低。Objective To estabhsh a method of HPLC to determine fumonisin B1（FB1） in maize and to get a general idea of the contamination of FB, in the samples of maize in Liaoning Province. Methods Chromatography conditions： C18 column,mobile phase was methanol： NaH2PO4,fluorescence detector. Results The recovery rate of spiked maize was between 71.77%-102.68% ,and mean recovery rate was 84.72%. The FB, content in the 78 samples of maize was 0.74±0.05 μg/g. Conclusion HPLC method can be estabhshed to determine FB1. The result indicates FB1 contamination rate of maize in Liaoning is low.

关 键 词：伏马菌素B1 高效液相色谱 

分 类 号：R155.5[医药卫生—营养与食品卫生学]