具有灭螺活性的金钱松内生真菌JJ18发酵条件优化  

Optimization of fermentation conditions of endophyte JJ18 of Pseudolarix kaempferi Gord for molluscicidal effect

作　　者：郭尚彬[1] 陈钧[1] 承曦[1] 周晓坤[1] 杨逊[1] 

机构地区：[1]江苏大学药学院生药学研究室,镇江212013

出　　处：《中国媒介生物学及控制杂志》2008年第1期37-40,共4页Chinese Journal of Vector Biology and Control

基　　金：江苏省2004年度研究生创新计划项目(xm04-57)

摘　　要：目的通过改进发酵工艺提高金钱松内生真菌JJ18的杀螺活性。方法采用单因素和正交试验对发酵培养基及发酵条件进行优化。按照WHO的杀螺剂浸泡试验法,以杀螺率为主要衡量指标,同时考察发酵液的生物量,对金钱松内生真菌JJ18发酵条件进行优化。结果以马铃薯175g/L为氮源,初始pH值6.0,葡萄糖和蔗糖15g/L为碳源,磷酸二氢钾0.5mmol/L为代谢调节因子,接种量2%,250ml三角瓶装液量50ml,温度30℃,120r/min,发酵7d,杀螺活性最大提高16.7%。生物量与杀螺率存在一定的相关性。结论通过改变发酵工艺可以提高杀螺活性,金钱松内生真菌JJ18具有开发应用的潜力。Objective To improve the molluseieidal effect by optimization of fermentation medium of endophyte JJ18 from Pseudolarix kaempferi Cord. Methods The medium composition and the cultivation conditions were optimized by orthogonal and single factor experiment. The immersion method suggested by WHO was applied to determine the effect and the main assessment index was mortality of the snails and biomass of fermantation liquid. Results This optimum culture medium included potato of 175 g/L, initial pH 6.0, glucose of 15 g/L, sucrose of 15 g/L, KHzPO4 of 0.5 mmol/L, the inoeulum dose of 2% , the medium content of 50 ml, temperature of 30 ℃ , incubation of 7 d and 120 r/min. The mortality of the snails was 100 % with the optimum culture medium, which was increased by 16.7 %. There exists a certain correlation between biomass of endophyte JJ18 and mortality. Conclusion The mortality is improved with optimization of fermentation techniques, so endophyte JJ18 of Pseudolarix kaempferi Gord has potential for molluseieidal application.

关 键 词：钉螺 内生真菌 杀螺作用 金钱松 JJ18 发酵工艺 

分 类 号：R184.38[医药卫生—流行病学]