^(60)Coγ辐照对莱芜姜当代生长的影响  

BIOLOGICAL EFFECTS OF ^(60)Co γ-IRRADIATION ON LAIWU GINGER VM_1 GROWTH

作　　者：周明[1] 黄金丽[1] 韦玉霞[1] 关秋竹[1] 张振贤[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094

出　　处：《核农学报》2008年第1期18-22,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家科技支撑计划项目(2006BAD13B06);农业部结构调整重大技术研究专项(04-06-02B)资助

摘　　要：本试验以莱芜姜根茎为材料,用60Coγ射线进行了02、0、40和60Gy 4个不同剂量的辐照处理,研究结果表明:辐照使莱芜姜当代(VM1)成苗率降低,出苗、展叶时间延迟,生长受到明显抑制,且随剂量的增加,其抑制作用增强;对辐照当代植株进行POD、EST同工酶和RAPD检测分析,均得到了与对照不同的差异性谱带,说明辐照生姜根状茎可以使植株在分子水平上发生变异;莱芜姜根茎使用LD30~40的中等剂量比较合适,适宜辐照剂量为20~30 Gy。Rhizome of Laiwu ginger were treated with T-irradiation at the doses of 0, 20, 40 and 60 Gy. The results showed that 60 Co T-irradiation inhibited the rhizome burgeoning, and decreased the survival rate of the seedlings, rate of leafexpansion and the growth of plants （VM1）. The inhibition effects became stronger with the increase of the irradiation dose. Different bands were found through the analysis of POD, EST isozymes and RAPD of VM1 plants , which showed that variation on molecular level occurred in VM1 plants. LD30-40 was appropriate for the irradiation of rhizomes of Laiwu ginger and the optimal irradiation dose was about 20 - 30 Gy.

关 键 词：姜 根茎 辐照效应 RAPD 同工酶 

分 类 号：S632.9[农业科学—蔬菜学]