鸡传染性支气管炎病毒澳大利亚T株S1全基因的克隆与序列分析  

Cloning and sequence analysis of S1 gene of Australian T strain of infectious bronchitis virus

作　　者：陈红英[1] 李新生[1] 崔保安[1] 张红英[1] 夏平安[1] 崔沛 廖仲磊[1] 

机构地区：[1]河南农业大学牧医工程学院 [2]河南省兽医防治站,河南郑州450008

出　　处：《西北农林科技大学学报（自然科学版）》2008年第2期12-16,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家“十五”食品安全重大攻关专项(2001BA804A30-11)

摘　　要：【目的】研究鸡传染性支气管炎病毒（IBV）澳大利亚T株S1基因全序列。【方法】采用RT-PCR技术，对IBVT株S1基因进行扩增、克隆和测序。【结果】测序结果表明，IBVT株S1基因大小为1739bp。与Gen—Bank中的29株IBV S1基因进行比较发现，IBVT株与吉林疫苗株（JAAS）S1基因核苷酸同源性为99．8％，有4个核苷酸的差异，其中有3个碱基发生非同义突变，氨基酸同源性为99．4％；与其余28株IBVs1基因核苷酸同源性为73．8％～84．4％，氨基酸同源性为66．8％～85．9％。进化分析发现T株与JAAS株之间的亲缘关系很近，与其他IBV株的亲缘关系均较远。【结论】JAAS株是T株的变异病毒，IBVT株的变异病毒在我国存在。[Objective] The research is to study S1 glycoprotein gene nucleotide sequence of infectious bronchitis virus（IBV）Australian T strain. [Method] S1 glycoprotein gene of IBV T strain was amplified by reverse transcription-polymerase chain reaction（RT-PCR）, then was cloned and sequenced. [Result] The sequencing result showed that the sequence consisted of 1 739 bp. When the nucleotide sequence of S1 gene of T strain was compared with that of 29 strains of IBV in GenBank,homology between T strain and JAAS strain was 99.8% at nucleotide level,only four nucleotide differences （A^498G,C^974T,T^1 251A,G^1 396T） were found,leading to three amino acids substitution （F^325 S, Q^417 H, S^466 A）, and the deduced amino acid homology was 99.4%. However,nucleotide homologies with other strains ranged between 73. 8% to 84.4% and the deduced amino acid homologies ranged between 66.8% -85.9 %. Evolution analysis indicated that the T strain was closely related to JAAS, very colse to the other strains of IBV. [Conclusion] The result showed that JAAS strain was a variant virus of T strain and there was a variant virus of IBV T strain in our country.

关 键 词：传染性支气管炎病毒 澳大利亚T株 S1基因 序列分析 

分 类 号：S851.31[农业科学—预防兽医学]