薰衣草薄荷叶非精油组分清除自由基作用的研究  

Study on scavenging free radical of the components of nonessential oils in Lavender mint leaves

作　　者：张军[1] 陈季武[1] 袁萌芽[1] 姚雷[2] 周影[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]上海交通大学植物科学系,上海201101

出　　处：《西北农林科技大学学报（自然科学版）》2008年第2期159-164,共6页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：【目的】为薰衣草薄荷叶非精油组分的医疗、保健和营养等功能提供理论依据。【方法】分别采用水、甲醇-水、乙酸乙酯和乙醇提取法对薰衣草薄荷叶非精油组分进行了提取,并分别采用超氧阴离子自由基(O2.-)、羟自由基(.OH)、过氧亚硝基(ONOO-)化学发光体系和.OH氧化损伤DNA的化学发光体系以及二苯代苦味肼基自由基(DPPH.)比色体系,对得到的4种提取物抗氧化能力进行比较。【结果】薰衣草薄荷叶非精油组分的有效成分主要存在于极性和弱极性溶剂提取物中。4种薰衣草薄荷叶非精油组分提取物不仅能够直接有效地清除O2.-、.OH、ONOO-和DPPH.,而且能够有效地抑制和延缓.OH对DNA的氧化损伤。【结论】薰衣草薄荷叶非精油组分是一种有效的、多功能的天然抗氧化剂和自由基清除剂,具良好的应用前景。[Objective] The stu er functions of components of non essential oils in tion, ethyl aceta Lavender mint lea te extraction and dy provides a theoretical basis for medical care, health, nutrition and othessential oils in Lavender mint leaves. [Method] The components of nonyes are distilled respectively by water extraction,methanol-water extracethanol extraction, then compared and evaluated by Chemiluminescence systems of superoxide anion free radical, hydroxyl radical, peroxynitrite and DNA oxidative damage caused by hydroxyl radical and colorimetric systems of DPPH free radical. [Result] The main active ingredients of components of nonessential oils in Lavender mint leaves exist in polar and weak polar solvent extracts. The experiments reveal that the four different extractions of the components of nonessential oils in Lavender mint leaves can not only directly scavenge superoxide anion free radical, hydroxyl radical, peroxynitrite and DPPH free radical, but also has a better ability to obviously mitigate and delay DNA oxidative damage caused by hydroxyl radical. [Conclusion] The components of nonessential oils in Lavender mint leaves are very effective and multifunctional crude antioxidants and free radical scavengers with a good application prospect.

关 键 词：薰衣草薄荷 非精油组分 自由基 DNA损伤 抗氧化剂 

分 类 号：TS202.3[轻工技术与工程—食品科学] Q505[轻工技术与工程—食品科学与工程]