电磁噪声阻断1800MHz手机辐射所致的晶状体上皮细胞内活性氧增加及DNA损伤  

Blocking 1800 MHz mobile phone radiation-induced reactive oxygen species production and DNA damage in lens epithelial cells by noise magnetic fields

作　　者：吴炜[1] 姚克[1] 王凯军[1] 鲁德强[2] 何继亮 徐立红 孙文均[2] 

机构地区：[1]浙江大学医学院附属第二医院眼科中心,浙江杭州310009 [2]浙江大学医学院浙江省生物电磁学重点研究实验室 [3]劳动卫生与环境卫生研究所 [4]生物化学与遗传学系,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2008年第1期34-38,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助项目(30570439)

摘　　要：目的:探讨1800 MHz手机辐射对人晶状体上皮细胞内活性氧(ROS)和DNA损伤的影响,以及叠加电磁噪声后对该效应的阻断作用。方法:体外培养的人晶状体上皮细胞受比吸收率(specific absorption rate,SAR)为4 W/kg的1800 MHz手机频段间断辐射24 h或与2μT电磁噪声联合作用24 h后,利用荧光探针检测ROS水平及用彗星试验检测DNA损伤水平。结果:4 W/kg手机辐射可明显诱发人晶状体上皮细胞内ROS及DNA损伤(P<0.001);与2μT电磁噪声联合暴露后可明显阻断该效应,与手机单独辐射相比,差异有显著性(P<0.001),但与假辐照组比较差异无显著性(P>0.05)。结论:电磁噪声可阻断1800 MHz手机辐射所诱发的晶状体上皮细胞内ROS及DNA损伤。Objective： To investigate whether the exposure to the electromagnetic noise can block reactive oxygen species （ROS） production and DNA damage of lens epithelial cells induced by 1800 MHz mobile phone radiation. Methods. The DCFH-DA method and comet assay were used respectively to detect the intracellular ROS and DNA damage of cultured human lens epithelial cells induced by 4 W/kg 1800 MHz mobile phone radiation or/and 2 tμT electromagnetic noise for 24 h intermittently. Results： 1800 MHz mobile phone radiation at 4 W/kg for 24 h increased intracellular ROS and DNA damage significantly （P〈0. 05）. However,the ROS level and DNA damage of mobile phone radiation plus noise group were not significant enhanced （P〉 0. 05） as compared to sham exposure group. Conclusion： Electromagnetic noise can block intracellular ROS production and DNA damage of human lens epithelial cells induced by 1800 MHz mobile phone radiation.

关 键 词：噪声/副作用 活性氧 DNA损伤 电磁场 彗星试验 辐射 晶体/细胞学 上皮细胞/代谢 

分 类 号：R779.1[医药卫生—眼科]