丹参酮ⅡA对人胃癌BGC-823细胞增殖及凋亡的影响  

Effects of Tanshinone Ⅱ A on Proliferation and apoptosis of the Human Gastric Cancer Cell Line BGC-823

作　　者：王旭[1] 颉玉欣[2] 单保恩[3] 

机构地区：[1]北京积水潭医院检验科,北京100035 [2]河北医科大学第三医院实验中心,河北石家庄050017 [3]河北医科大学第四医院科研中心,河北石家庄050017

出　　处：《癌变．畸变．突变》2008年第1期56-59,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的：探讨丹参酮ⅡA（tanshinoneⅡA，TanⅡA）对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法：采用0～10μg／ml Tan ⅡA分别作用于BGC-823细胞48h及72h，MTT比色法观察药物对细胞生长的抑制效应；2、5、10μg／ml TanⅡA分别作用于细胞72h，应用HE染色、DNA琼脂糖凝胶电泳、流式细胞术分析药物对细胞凋亡的影响。结果：TanⅡA明显抑制BGC-823细胞增殖、诱导细胞凋亡，镜下可见BGC-823细胞明显的凋亡特征性改变；5、10μg／ml Tan ⅡA处理组细胞DNA电泳可见典型的“梯状”条带；FCM结果显示5、10μg／ml TanⅡA处理组细胞，凋亡率分别为（20．60±1．84）％、（31．03±1．47）％，与对照组（5．23±0．39）％比较差异有统计学意义（P〈0．01）。结论：在一定条件下，TanⅡA可抑制人胃癌BGC-823细胞增殖，诱导其凋亡。BACKGROUND AND AIM： F/fleets of tanshinone Ⅱ A （Tan Ⅱ A） on proliferation and apoptosis of the human gastric cancer cell line BGC-823 were studied. MATERIALS AND METHODS： The inhibitory effect on proliferation of BGC-823 cells was evaluated in vitro by using MTT colorimetric assay after cells were treated with Tan Ⅱ A at various concentrations （0-10μg/ ml） for 48 h and 72 h. Cell apoptosis was analyzed by HE staining, DNA agarose gel eleetrophoresis and flow cytometry after BGC-823 cells were treated with 2, 5, 10 μg/ml Tan Ⅱ A for 72 h. RESULTS： Tan Ⅱ A inhibited the proliferation of BGC-823 cell markedly. The typical morphological features indicative of apoptotic state were seen under the light microscope. The DNA ladder was generated after treatment with Tan Ⅱ A at the concentrations of 5 and 10 μg/ml.The apoptotic rates were （20.60±1.84）% and （31.03±1.47）% in BGC-823 cells treated with 5, 10μg/ml of Tan Ⅱ A, respectively. There was marked difference compared with the control group whose apoptotic rate was （5.23±0.39）%. CONCLUSION： Tan Ⅱ A could inhibit proliferation and induce apoptosis of human gastric cancer ceil line BGC-823 in this test.

关 键 词：丹参酮ⅡA 人胃癌BGC-823细胞株 细胞凋亡 

分 类 号：R730.45[医药卫生—肿瘤]