以胎鼠为供体的肾上腺移植实验研究  

Adrenal allografts using fetal rats as donors

作　　者：田顺亮[1] 孙建森[2] 

机构地区：[1]桂林医学院基础医学院人体解剖学教研室,广西桂林541004 [2]第三军医大学基础医学部人体解剖学教研室,重庆市生物力学实验室,重庆400037

出　　处：《第三军医大学学报》2008年第3期254-256,共3页Journal of Third Military Medical University

摘　　要：目的研究以不同胎龄胎鼠为供体的肾上腺移植后,移植物的结构重建及分泌功能状况。方法将30只SD大鼠,分为5组,每组6只。A组,假手术组;B组,双侧肾上腺切除组;C、D、E组分别以16、18、20d龄胎鼠为供体进行移植。结果术后4周,C、D、E组镜下显示被膜增厚,被膜下有许多新增殖的细胞,球状带、束状带有较好的重建。体质量:A组术后第3周降至最低点,第4周逐渐恢复接近术前水平;B组术后持续上升;C、D组术后前2周增高,后2周持续下降;E组术后前3周缓慢升高,第4周回落。血清皮质酮水平:B组术后急剧下降;C、D、E组术后2周都大幅下降,然后逐渐增高;D、E组术后同期水平接近,C组术后水平却明显低于D、E组(P<0.05)。结论胎鼠肾上腺移植后易于成活,结构可较好地重建,分泌功能逐步增强,是一种合适的供体;大龄胎鼠的供体移植后,在免疫排斥反应方面与小龄胎鼠的供体无明显差异,而结构重建及分泌功能优于小龄胎鼠的供体。Objective To study the reconstruction and secretory function of the grafts after adrenal transplantation using fetal rats as donors. Methods Thirty adult SD rats randomly received sham operation （group A） , excision of bilateral adrenals （ groups B, C, D and E） , and additionally adrenal transplantation in groups C, D and E using fetal rats aged 16, 18 and 20 days respectively as donors. Results The weight of group A got down to the lowest at the 3rd week, and restored to the preoperation level at the 4th week after sham operation. The weight of group B gradually rose up within 4 weeks. The weight of groups C and D rose up in the first 2 weeks, then down. The weight of group E rose slowly within the first 3 weeks, then down. The serum level of corticosterone decreased sharply in group B after operation, while that of groups C, D and E went down obviously in the first 2 weeks, then up, but that in group C was significantly lower than that in groups D and E. Microscopically, we found the capsule of allografts thicken and underneath many proliferative cells and well-constructed glomerular and fascicular zone 4 weeks after operation. Conclusion After transplantation, the fetal adrenal could survive and reconstruct well, and its secretory function gets better gradually. The graft from the elder donor is better than that from the younger in reconstruction and secretory function, but shows no obvious difference in immunological rejection.

关 键 词：胎鼠 同种异体肾上腺移植 皮质酮 

分 类 号：R-332[医药卫生] R617