血清1和3型多杀性巴氏杆菌铁调控外膜蛋白共同抗原的初步分析  

Primary Study on Common Antigens of Iron-Regulated Outer Membrane Proteins of Serotype 1 and 3 Pasteurella multocida Strains

作　　者：余旭平[1] 邬栋栋[1] 刘晓宁[1] 胡东建[1] 杜鑫[2] 陈玉银[2] 

机构地区：[1]浙江大学动物预防医学研究所,杭州310029 [2]浙江大学应用生物资源研究所,杭州310029

出　　处：《畜牧兽医学报》2008年第2期245-250,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30571380)资助

摘　　要：用普通BHI和缺铁BHI培养基培养禽多杀性巴氏杆菌C48-19(血清1型)、P1059株(血清3型),提取外膜蛋白(OMPs),SDS-PAGE电泳比较不同培养条件下两株细菌的外膜蛋白,发现缺铁培养时2株细菌均增加了87、81、79、64、60.5 ku铁调控外膜蛋白(IROMPs)。以C48-19株攻毒后耐过的鸡血清作为抗体进行免疫印迹,检测到其中的87、79和60.5 ku蛋白是2株细菌共同的、具有交叉免疫反应性的IROMPs,推测这3种蛋白可能是这2个菌株的共同抗原,并可能在C48-19感染的鸡体内特异表达;对鸡胚尿囊液中培养的C48-19株OMPs的SDS-PAGE电泳和免疫印迹试验,也观察到类似IROMPs的表达。这些IROMPs的发现为亚单位疫苗的研制提供了候选蛋白。Avian Pasteurella multocida strain C48-19 （serotypel）, P10s9 （serotype3） grown in brainheart infusion broth containing the iron chelator dipyridyl expressed additional outer membrane proteins （OMPs） compared with those grown in normal brain-heart infusion. These OMPs were merely expressed in iron deficient medium and were considered to be iron-regulated outer membrane proteins （IROMPs）. The SDS-PAGE patterns showed the existence of 87, 81, 79, 64, 60.5 ku IROMPs in both strains. The following Western blotting demonstrated that 87, 79, 60.5 ku proteins can be recognized by the convalescent serum from chicken recovered form C48-19＇s infection. These three proteins were considered to be conserved IROMPs with cross immunoreactivity, and might be common antigens of these two strains that expressed in vivo. Furthermore, OMPs of C48-19 grown in chicken allantolic fluid shared similar pattern with those grown in iron depleted media.

关 键 词：禽多杀性巴氏杆菌 铁调控外膜蛋白 SDS-PAGE 免疫印迹 

分 类 号：S852.61[农业科学—基础兽医学]