肝泰胶囊抗肝纤维化作用的实验研究  

Effect of gantai capsule on hepatic fibrosis in rats

作　　者：刘红艳[1] 虞涤霞 段瑞娴[1] 程勇卫[1] 吴翠环[3] 林菊生[1] 唐望先[1] 

机构地区：[1]华中科技大学同济医学院同济医院肝病研究所,湖北武汉430030 [2]武汉市七医院 [3]华中科技大学同济医学院病理科

出　　处：《胃肠病学和肝病学杂志》2008年第2期163-166,169,共5页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨肝泰胶囊对CCl4诱导的肝纤维化大鼠的作用及其抗肝纤维化的可能机制。方法将40只Wistar大鼠随机分为正常对照组、肝纤维化模型组、肝泰组及肝复乐组。采用光镜、电镜观察肝组织病理学改变,Von Gieson(VG)染色及胶原面积半定量分析反映各组肝纤维化程度。采用放射免疫法测定血清层粘连蛋白(LN)、Ⅲ型前胶原(PC-Ⅲ)、Ⅳ型前胶原(Ⅳ-C)、透明质酸(HA)的含量,同时用高效液相色谱-电化学(HPLC-ECD)法检测肝组织中去甲肾上腺素(Norepine phrine,NE)的水平。结果肝泰组较模型组比:肝组织肝纤维化程度明显改善;②肝组织胶原纤维面积明显减少;③血清透明质酸和层粘连蛋白显著降低;④肝功能损伤明显减轻;⑤组织中NE的含量明显高于模型组。结论肝泰对慢性肝损伤有一定的保护作用。Objective To investigate the possible mechanism of gantai capsule resisting hepatic fibrosis induced by carbon tetrachloride （CCl4）. Methods Fourty rats were randomly divided into control group, model group, ganfule and gantai treated group. Light and electron microscope were adopted to observe the histopathology of the hepatic tissue, VG dying and semiquantitation of collogen area were used to reflect the degree of fibrosis, and LN, PC- Ⅲ , HA, Ⅳ-C in the serum were evaluated to reflect the level of hepatic fibrosis. Meanwhile, The NE concentrations in liver tissue were determined with high performance liquid chromatography-electrochemical detector （HPLC-ECD）. Results Compared with model group, gantai treated group revealed： ① The degree of fibrosis was improved ② Liver collagen fibers markedly was decreased. ③ Serum hyaluronic acid and laminin was obviously decreased. ④ Liver function was improved. ⑤ The concentrations of NE in liver tissue were elevated. Conclusions Gantai has protective effect on chronic liver injury in rats.

关 键 词：肝泰 肝纤维化 去甲肾上腺素 高效液相色谱-电化学 

分 类 号：R575[医药卫生—消化系统]