人胎肝细胞移植治疗代谢性疾病的免疫学研究  

Immunology research on human fetal liver cells transplantation in treatment of metabolic diseases

作　　者：万江波[1] 刘晓莉[1] 梁辉[1] 

机构地区：[1]上海交通大学医学院新华医院血液科,上海200092

出　　处：《上海交通大学学报（医学版）》2008年第1期69-71,共3页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的利用人胎肝细胞移植治疗小鼠α-L-艾杜糖苷酶(IDUA)缺陷引起的黏多糖贮积症(MPS),观察移植后受体的免疫反应、血清及肝脏IDUA活性恢复情况。方法将已鉴定纯化的人胎肝细胞移植给IDUA缺陷小鼠,在移植前及移植后第5、10、15天,流式细胞仪检测受体小鼠T细胞亚群CD3、CD4和CD8;ELISA法检测免疫指标IL-2、TNF-α、IFN-γ;荧光分光光度计法检测血清及肝脏IDUA活性情况。结果胎肝细胞移植后能定植在受体肝脏中,受体在移植后第5、10、15天分别与移植前比较,血清中反映免疫状态的指标T细胞亚群、IL-2、TNF-α和IFN-γ均显著升高(P<0.05);血清及肝脏IDUA活性在移植后第5天显著提高(P<0.01),以后随着时间的延长,活性随之衰减。结论人胎肝细胞移植给IDUA缺陷小鼠后,能够定植在受体肝脏中。然而由于免疫排斥反应,IDUA活性在移植后第5天达到高峰,但在第10天后下降至移植前水平。Objective To investigate the immunological reaction, serum and liver α-L-iduronidase （IDUA） activity after human fetal liver cells （FLCs） transplantation therapy on mucopolysaccharidosis （MPS） which attribute to IDUA defect. Methods FLCs were transplanted into IDUA deficiency mice. T cell subsets （CD3, CD4 and CD8） was detected by flow cytometry, while IL-2, TNF-α and IFN-γ by ELISA, and serum and liver IDUA activity by fluorospectrophotometer at different stage（ before transplantation and 5, 10, 15 days after transplantation）. Results Human FLCs survived in IDUA deficiency mice, bringing elevated serum and liver enzyme activity to receptor. T cell subsets, IL-2, TNF-α and IFN-γ was significantly higher 5, 10 and 15 days after transplantation than those before transplantation （ P 〈 0.05）. Five days after transplantation, IDUA activity reached the peak in all groups （ P 〈 0. 01 ） , while decreased gradually from then on. Conclusion Transplanted FLCs into IDUA deficiency mice can be found in receptor liver. Due to immunological rejection, serum and liver enzyme activity may reach the peak 5 days after transplantation and drop to levels of pretransplantation 10 days after transplantation.

关 键 词：胎肝细胞 移植 黏多糖贮积症 免疫抑制剂 

分 类 号：R589[医药卫生—内分泌]