口腔鳞状细胞癌cyclin E及p21^waf1蛋白定量检测及意义  

Quantitative Detection of Cyclin E and p21^(waf1) Proteins in Oral Squamous Cell Carcinomas and the Clinical Significance

作　　者：蔡扬[1] 何园[2] 卢虹[1] 付伟[1] 李咏[1] 

机构地区：[1]贵阳医学院附属医院口腔诊疗中心,贵阳市550004 [2]同济大学附属口腔医院

出　　处：《中国肿瘤临床》2008年第3期142-144,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:39460139);教育部留学回国人员科研启动基金(编号:N2005-1);贵州省政府省长专项基金资助(编号:S2004-16)

摘　　要：目的:探讨口腔鳞状细胞癌(oralsquamous cell cancer,OSCC)cyclin E、p21waf1蛋白表达与抑癌基因p53突变的关系及其临床病理学意义。方法:采用流式细胞术(FCM)对8例正常口腔粘膜及30例OSCC石蜡包埋组织中cyclin E和p21waf1蛋白表达进行定量检测,分析其与突变型p53蛋白表达及临床病理学参数间的关系。结果:OSCC组织cyclinE蛋白平均表达量高于正常口腔粘膜(t=-6.582,P<0.01),过表达率为63.33%(19/30);而p21waf1蛋白平均表达量低于正常口腔粘膜(t=8.126,P<0.01),低表达率为53.33%(16/30);OSCC组织cyclinE/p21waf1比值高于正常口腔粘膜(t=-9.434,P<0.01),异常率为100%(30/30);p53阳性组p21waf1表达量低于p53阴性组(t=3.905,P<0.01),两者间呈负相关(r=-0.491,P<0.05)。p21waf1表达量在OSCC有淋巴结转移组低于无淋巴结转移组(t=3.391,P<0.01),但与OSCC分化无关(P>0.05);而cyclinE表达量与OSCC肿瘤分化及淋巴结转移均无关(P>0.05)。结论:cyclinE蛋白表达上调及p21waf1蛋白表达下调是OSCC常见异常现象,p21waf1蛋白表达下调可能与p53突变有关,并与OSCC淋巴结转移密切相关,G1/S期正负调控因子cyclinE/p21waf1比例失衡在OSCC发生中可能起着重要作用。Objective： To detect cyclin E and p21^waf1 proteins quantitatively in oral squamous cell carcinomas（OSCC）, to investigate the correlation between these expression levels and the expression of mutated p53 protein, and to determine the significance of these parameters in tumor differentiation and lymph node metastasis of OSCC. Methods： Flow cytometry was employed to quantitatively detect the expression of cyclin E and p21^waf1 in 8 samples of normal oral epithelium and 30 samples of OSCC. The expression of mutated p53 protein was detected by immunohistochemistry. Using SPSS12.0, we analyzed the correlation between the expression of p21^waf1 and p53 proteins and examined the relationship between these levels and tumor differentiation and lymph node metastasis of OSCC. Results： The expression of cyclin E protein in OSCC was higher than that in the normal oral epithelium （t=-6.582,P〈0.01）, and 63.33%（19/30） of the OSCC patients presented with upregulated cyclin E. The expression of p21^waf1 protein was lower in OSCC than in the control group （t=8.126,P〈0.01）, and 53.33%（16/30） of the OSCC patients presented with downregulated p21^waf1. The ratio of cyclin E/p21^waf1 in OSCC was significantly higher than in the normal group （t=-9.434,P〈0.01）. The expression of p21^waf1 protein was lower in OSCC tissues with positive p53 staining than in those without p53 staining （t=3.905,r=-0.491,P〈0.01）. p21^waf1 protein downregulation was significantly associated with lymph node metastasis, while no correlation was found between cyclin E expression and tumor differentiation or lymph node involvement （P〉0.05）. Conclusion： OSCC patients have up- regulated cyclin E and downregulated p21^waf1. The downregulation of p21^waf1 protein may partly be a result of p53 mutation, which probably plays a role in lymph node metastasis of OSCC. The imbalance between the positive and negative regulators of G1/S caused by cyclin E upregulation and p21^waf1 downregulation might be involved in OSCC.

关 键 词：口腔鳞状细胞癌 细胞周期素E P21^WAF1 p53流式细胞术 

分 类 号：R739.8[医药卫生—肿瘤]