中国小黑豆抗源对大豆孢囊线虫4号生理小种抗病的生化反应  

Biochemical Responses of Resistance to Race 4 of Heterodera glycines in Chinese Black Soybean

作　　者：颜清上[1] 王连铮[1] 陈品三[2] 

机构地区：[1]中国农业科学院作物育种栽培研究所,北京100081 [2]中国农业科学院植物保护研究所,北京100094

出　　处：《作物学报》1997年第5期529-537,共9页Acta Agronomica Sinica

摘　　要：抗源品种灰皮支黑豆、元钵黑豆和高感品种鲁豆1号为供试大豆材料,大豆孢囊线虫4号生理小种卵和二龄幼虫为接种物。接种后17天,测定大豆根部的营养物质和次生代谢物含量。与不接种对照比,抗源品种灰皮支黑豆和元钵黑豆根部的总糖含量分别降低24.91％和37.77％,而感病对照鲁豆1号升高46.01％,果糖和麦芽四糖含量在感病对照根上增加幅度较大,分别为144.30％和62.90％,但在抗源品种根上或是具有较小幅度的增加,或是表现降低。接种与否,感病对照根上的游离氨基酸总量以及精氨酸、谷氨酸、丙氨酸、天门氨酸、亮氨酸和缬氨酸含量增加的百分数明显高于抗源品种。胱氨酸含量在两个抗源品种上始终为0。接种后,抗感品种根部的总酚含量、绿原酸含量和阿魏酸含量都增加,但抗源品种增加量比感病品种鲁豆1号高出一倍到数倍;类黄酮含量和木质素含量变化在抗感品种间正好相反:抗源品种含量增加,感病品种却为降低。The contents of nutriment and some secondary metabolites in fibrous roots of soybean seedlings were determined at 17 days after inoculation with race 4 of Heterodera glycines. The results showed as follows; The content of total sugar in infected roots of susceptible control, Ludou 1, was 46. 01% higher than that in healthy one, meanwhile the contents of total sugar were 24. 91% and 37. 77% lower in the roots of two resistant varieties, Huipizhihei-dou and Yuanboheidou, respectively. Compared to their corresponding healthy roots, the contents of fructose, maltotetrose increased 144. 30% and 62. 90% in infected roots of cultivar Ludou 1 respectively, whereas they decreased or increased to a smaller extent in the roots of resistant cultivars. After inoculation, the increasing percentages of the concentrations of total free amino acid as well as arginine, glutamic acid, alanine, aspartic acid, leucine, valine in roots of cultivar Ludou 1 were higher than that in the roots of two resistant cultivars. The contents of cystine either in infected roots or in healthy roots of the two resistant cultivars were all zeros. In response to inoculation, the increases of the contents of total phenolics, chlorogenic acid and fer-ulic acid in the roots of resistant cultivars, Huipizhiheidou and Yuanboheidou, were one time to several times more than these in the roots of susceptible control, Ludou 1. At the same time, the contents of flanvonoid and lignin varied diversely between resistant cultivars and susceptible one, which increased in infected roots of resistant cultivars and decreased in infected roots of Ludou 1 after inoculation.

关 键 词：大豆 孢囊线虫 抗源品种 糖分 游离氨基酸 

分 类 号：S565.103.4[农业科学—作物学]