内质网分子伴侣家族成员在幽门螺杆菌感染者胃黏膜中的表达和意义(英文)  

Expression of endoplasmic reticulum molecular chaperon in gastric mucosa infected with Helicobacter pylori

作　　者：王孟春[1] 郭静波[1] 刘明华[1] 高娜[1] 方文刚[2] 李岩[1] 

机构地区：[1]中国医科大学附属盛京医院消化内科,辽宁沈阳110004 [2]中国医科大学发育生物学研究室,辽宁沈阳110001

出　　处：《中国现代医学杂志》2008年第2期129-132,共4页China Journal of Modern Medicine

摘　　要：目的探讨内质网分子伴侣家族成员Grp94、Grp78和PDI在幽门螺杆菌感染者胃黏膜组织中的表达及意义。方法应用半定量RT-PCR方法及Westernblot方法分别检测感染与未感染幽门螺杆菌的人胃黏膜组织中Grp94、Grp78和PDI的mRNA及蛋白的表达情况。结果未感染组28例,Grp94、Grp78和PDI的mRNA表达量分别为(0.427±0.0360)、(0.8038±0.500)和(0.5198±0.0379),感染组32例,Grp94、Grp78和PDImRNA表达量为(0.882±0.082)、(1.1586±0.0839)和(1.0642±0.1533)。两组相比,感染组的Grp94、Grp78和PDImRNA的表达量明显高于未感染组(P<0.01);未感染组Grp94、Grp78和PDI蛋白表达量分别为(0.427±0.0360)、(0.4345±0.0545)和(0.5198±0.0379),感染组Grp94、Grp78和PDI蛋白表达量为(0.671±0.072)、(0.6204±0.0412)和(0.8252±0.032)。两组相比,感染组Grp94蛋白的表达量明显高于未感染组(P<0.01)。结论幽门螺杆菌感染可使人胃黏膜增加Grp94、Grp78和PDImRNA的表达及蛋白的合成,这可能有利于胃黏膜的自身保护作用。[Objective] To study the expression of endoplasmic reticulum molecular chaperon in gastric mucosa infected with Helicobacter pylori. [Methods] Semi-quantitative RT-PCR method was used to examine the mRNA expression of Grp94, Grp78 and PDI in H. pylori infected and non-infected gastric mucosa. Western blot method was used to examine the expression level of Grp94, Grp78 and PDI protein in infected and uninfected gastric mucosa. [Results] The expression of Grp94 mRNA, Grp78 mRNA and PDI mRNA were （0.424±0.055）, （0.8038±0.0500）, （0.5704±0.0794） in uninfected group（28 cases） respectively; Grp94 mRNA, Grp78 mRNA, PDI mRNA were （0.882±0.082）, （1.1586±0.0839）, （1.0642±0.1533） in infected group（32 cases） respectively. The expression amounts of Grp94, Grp78 and PDI protein were （0.427±0.0360）, （0.4345±0.0545）, （0.5198±0.0379） in uninfected group. In infected group the expression of protein amounts of Grp94, Grp78, PDI were （0.671±0.072）, （0.6204±0.0412）, （0.8252±0.032） respectively. The expression amounts of Grp94 mRNA, Grp78 mRNA, PDI mRNA and Grp94, Grp78 and PDI protein in infected group showed great increase （P 〈0.01） respectively. [Conclusions] Helicobacter pylori infection may increase the expression of Grp94, Grp78 and PDI at mRNA level in gastric mucosa, meanwhile, it also increases the synthesis of orotein.

关 键 词：幽门螺杆菌 内质网分子伴侣 

分 类 号：R573.3[医药卫生—消化系统]