短期酒精染毒对大鼠学习记忆及脑组织神经生长因子表达的影响  

Effects of short-term alcohol intragastric administration on rat learning and memory and the expression of NGF in brain

作　　者：朱旭阳[1] 许亚军[2] 吴茂旺[1] 陈宗云[1] 

机构地区：[1]皖南医学院法医学系,芜湖241002 [2]皖南医学院心理学系

出　　处：《中国行为医学科学》2008年第2期117-119,共3页Chinese Journal of Behavioral Medical Science

摘　　要：目的探讨短期酒精染毒对大鼠学习记忆的影响，通过免疫组织化学方法检测大鼠大脑皮层、海马和丘脑神经生长因子（NGF）的分布与表达，探讨其意义。方法63只成年雄性SD大鼠随机平均分为3组：对照组，低浓度染毒组（LAA组），高浓度染毒组（HAA组）。对照组以生理盐水灌胃，LAA组和HAA组分别以每天每只5ml／kg和10ml／kg标准用55％酒精灌胃。酒精灌胃3，7和14d后行Morris水迷宫实验检测大鼠学习记忆变化，取脑，采用免疫组织化学SABC染色法检测NGF分布及表达。在Olympus显微镜及图像分析系统下测定NGF阳性细胞数。结果LAA组染毒14d后，Morris水迷宫实验显示大鼠学习记忆功能损伤，HAA组染毒3，7和14d后，Morris水迷宫实验显示不同程度学习记忆功能损伤；LAA组染毒3d大脑皮层、海马CA3区和丘脑NGF免疫阳性细胞数[分别为（15．26±3．38）个，（5．88±1．76）个，（16．34±4．05）个]，与对照组生理盐水灌胃3d大脑皮层、海马CA3区和丘脑NGF免疫阳性细胞数[（14．45±3．09）个，（5．37±1．65）个，（15．76±4．09）个]比差异无显著性（P〉0．05），LAA组酒精染毒7、14d脑组织NGF免疫阳性细胞数[分别为（19．35±3．76）个，（10．36±2．08）个，（21．66±4．34）个和（23．65±3．87）个，（15．54±2．11）个，（24．43±4．45）个]和HAA组3，7和14d脑组织NGF免疫阳性细胞数[分别为（23．68±3．76）个，（14．87±2．09）个，（24．21±4．07）个；（26．64±4．46）个，（19．76±2．90）个，（28．71±4．93）个和（27．12±3．81）个，（19．27±2．38）个，（29．75±3．52）个]增加，与对照组生理盐水灌胃3d相比差异有显著性（P〈0．05）。结论短期酒精染毒可致大鼠学习记忆功能损伤，损伤程度与染毒的量和时间相关，其机制可能与短期酒精染毒改变大鼠脑NGF表达相关。Objective To explore the effects of short-term alcohol intragastric administration on learning and memory and the distribution and expression of NGF through immunohistochemistry method in cerebral cortex, hippocampus and cerebral ganglion of rat. Methods 63 adult male SD rats were randomly divided to 3 groups ： control group, low alcohol administration group （LAA group） and high alcohol administration group （HAA group）, control group were administrated by normal saline,different dosage of 55% alcohol （5ml · kg^-1· d^-1 and 10ml · kg^-1·d^-1） were respectively administrated to LAA and HAA group daily. After 3,7 and 14 clays,Morris water maze was used to detect the ch.anges of learning and memory. Distribution and expression of NGF were ob- served with immunohistochemical SABC staining. The NGF-immunoreactive cells numbers were detected by Olympus microscope and image analysis system. Results The impairments of learning and memory detected by Morris water maze trail were observed after alcohol intragastric administration 14 clays of LAA group, while different im- pairments of learning and memory were observed after alcohol intragastric administration 3,7 and 14 clays of MAA group. The numbers of NGF-immunoreactive cells in cerebral cortex, hippocampus and cerebral ganglion increased obviously after alcohol intragastric administration 7 and 14 clays of LAA group [ 7d ： 19.35 ±3.76,10.36 ± 2.08, 21.66 ±4.34 ; 14d ：23.65 ±3.87,15.54 ± 2.11,24.43 ± 4.45 ] and alcohol intragastric administration 3,7 and 14 days of HAA group [ 3 d： 23.68 ± 3.76,14.87 ± 2.09,24.21 ± 4.07 ; 7 d： 26.64 ± 4.46,19.76 ± 2.90,28.71 ±4.93 ; 14d ： 27.12 ± 3.81,19.27 ± 2.38,29.75 ±3.52 ] compared with control group which alcohol intragastric administration 3 days[ 3d： 14.45 ±3.09,5.37 ± 1.65,15.76 ±4.09 ] （P〈0.05） ,while it didn＇t change much after alcohol intragastric administration 3 clays of LAA group[ 3d： 15.26 ± 3.38,5.88 ± 1.76,16.34 ± 4.05 ] compared with control group which alcoh

关 键 词：酒精 学习记忆 神经生长因子 时间量效 

分 类 号：R749.6[医药卫生—神经病学与精神病学]