三氮唑席夫碱衍生物诱导人肝癌细胞SMMC-7721凋亡作用  

Apoptosis of Human Hepatocarcinoma Cell SMMC-7721 Induced by Triazole Schiff Base Derivatives

作　　者：马永超[1] 张维娟[1] 皇甫超申[1] 胡国强[2] 杨锐生[2] 刘彬[1] 

机构地区：[1]河南大学神经生物学研究所,河南开封475004 [2]河南大学药学院,河南开封475004

出　　处：《河南大学学报（自然科学版）》2008年第1期65-70,共6页Journal of Henan University:Natural Science

基　　金：河南省科技攻关项目(编号124170229)

摘　　要：用不同浓度的3-吡啶-3-基-4-[(4-羟基-3-甲氧基-苯亚甲基)氨基]-5-甲硫基-1,2,4-三唑(LH-37)处理细胞,观察其对人肝癌细胞SMMC-7721的凋亡诱导作用.结果发现,LH-37可抑制细胞增殖,且抑制率随浓度的增加而增高,IC50为2.0×10-4mol/L;药物浓度为1×10-5mol/L时作用48 h,SMMC-7721细胞皱缩,呈现凋亡各期改变,Caspase-3阳性细胞明显增加,细胞凋亡率显著高于对照组的现象(P<0.05).The effect of 3-pyridine-3-yl-4-[（4-hydroxy-3-methoxy-benzylidene）amino]-5-methylthio-4H-[1,2, 4] triazole （LH-37） on induction of apoptosis in hepatocarcinoma cell SMMC-7721 was observed . Following the increase in the concentration of LH-37, the inhibition of the cell proliferation was enhanced significantly and the ICso was 2.0× 10^-4 mol/L; treatment of SMMC-7221 cells with 1×10^-5 mol/L LH-37 for 48 hours increased the percentage of active Caspase-3 positive cells from （ 10.7±0.8） % to （33.6±1.2） % （P〈0.05） and more apoptosis features of cells were observed in LH-37 treatment groups than control group. These results indicate that LH-37 can inhibit proliferation and induce apoptosis of the human hepatocarcinoma cell SMMC-7721.

关 键 词：三氮唑席夫碱衍生物 肝细胞癌 细胞增殖 细胞凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]