高效液相色谱法同时测定Pichia pastoris发酵过程中腺苷甲硫氨酸相关的六种物质  

Direct separation and determination of SAM and its analogs in Pichia pastoris by HPLC

作　　者：张建国[1] 王学东[1] 魏东芝[1] 

机构地区：[1]生物反应器工程国家重点实验室华东理工大学生物工程学院,上海200237

出　　处：《工业微生物》2008年第1期6-9,共4页Industrial Microbiology

摘　　要：建立了用高效液相色谱同时测定Pichia pastoris菌体中腺苷甲硫氨酸及其代谢相关物质(AMP,腺苷,腺嘌呤,SAH)。采用Hypersil SCX色谱柱(4.6 mm×250 mm,5μm),柱温是30℃,流动相是0.5 mol/L甲酸铵(用甲酸调至pH 4.0),流速为2.0 mL/min,检测波长是254 nm。结果表明该方法可以直接从细胞裂解液对六种物质进行分离和定量。该测定方法简便、可靠、准确、灵敏度高,有利于说明Pichia pastoris发酵产腺苷甲硫氨酸过程中的代谢情况。A method for determination of SAM and related compounds in Pichia pastoris by HPLC was established. The separation was performed with a Hypersil SCX column（4.6mm×250mm,5μm） at 30℃, and the mobile phase consisted of 0.5mmol/L ammonium formate （pH was adjusted to 4.0 using formic acid）. UV detection wavelength was 254nm. The good linearity was obtained during determination of SAM and its analogs. This method has the advantages of simple sample preparation, accuracy, sensitiveness and stability.

关 键 词：腺苷甲硫氨酸 高效液相色谱 PICHIA PASTORIS 

分 类 号：O657.72[理学—分析化学] TQ920.1[理学—化学]