紧密连接蛋白claudin-1在肠易激综合征患者肠黏膜中的表达及意义  

Expression of intestinal mucosa tight junction claudin-1 proteins in patients with irritable bowel syndrome

作　　者：孔武明[1] 李光[1] 龚均[2] 

机构地区：[1]焦作市人民医院,河南焦作454100 [2]西安交通大学第二医院

出　　处：《山东医药》2008年第2期27-29,共3页Shandong Medical Journal

基　　金：国家自然科学基金项目(30170414)

摘　　要：目的探讨肠易激综合征(IBS)患者肠黏膜紧密连接蛋白claudin-1表达在排便性状和习惯改变中的意义。方法用免疫组化方法检测对照组(痔疮10例、结肠息肉10例)和观察组(43例IBS患者,腹泻型23例、便秘型20例)小肠和结肠黏膜claudin-1表达。结果免疫组化定位显示两组肠黏膜claudin-1均分布于肠上皮紧密连接的细胞膜,胞核及核膜无表达;与对照组比较,观察组腹泻型患者小肠和结肠黏膜claudin-1表达明显下降(P<0.05),便秘型患者claudin-1表达明显上升高(P<0.05),腹泻型与便秘型比较有显著差异(P<0.05)。结论claudin-1表达异常在IBS患者排便性状和习惯改变形成中可能有重要作用。Objective To investigate the changes of intestinal mucosa tight junction （TJ） proteins claudin-1 in patients with irritable bowel syndrome （IBS） , and elucidate its possible roles in the changes of bowel evacuation habit and formation in these patients. Methods By immunohistochemical assay, TJ protein claudin-1 levels were detected in intestinal mucosa in control group, D-IBS （diarrhea IBS） group and C-IBS （constipation IBS） group. Results It was observed that claudin-1 was localized in the plasma membranes of epithelial cells, including the apical end of the epithelial cells. Rarely, the claudins were concentrated at the site of TJ only. Diffuse cytoplasmic staining was rarely observed. Compared with control group, claudin-1 protein levels in small intestinal mucosa and colonic mucosa membrane in D-IBS group were significantly decreased （ P 〈 0. 05 ）, and which in C-IBS group were significantly elevated （P 〈 0.05）. Conclusion Claudin-1 has a potential important role in the changes of bowel evacuation habit and formation in patients with IBS. It is not due to the location changes of claudins protein in TJ, but maybe caused by the quantitative changes of the expression of TJ protein.

关 键 词：肠易激综合征 紧密连接蛋白 CLAUDIN-1 

分 类 号：R574.4[医药卫生—消化系统]