月桂酸胶束电动毛细管色谱快速分离三嗪类农药的研究  

Rapid Determination of Triazine Herbicides in Water by Micellar Electrokinetic Chromatography Using Lauric Acid as a Surfactant

作　　者：李晓静[1] 黄丽涵[1] 徐远金[1] 

机构地区：[1]广西大学糖业工程技术研究中心,广西南宁530004

出　　处：《分析测试学报》2008年第2期174-177,共4页Journal of Instrumental Analysis

基　　金：广西科学基金资助项目(桂科能0718009-2E)

摘　　要：以月桂酸为表面活性剂和三羟甲基氨基甲烷（Tris）组成缓冲溶液，建立了毛细管胶束电动色谱快速测定8种三嗪类农药的方法。对电泳介质的种类及浓度、pH值、操作电压和进样时间等影响因素进行了优化，在以50mmol／L月桂酸和100mmol／L Tris作为缓冲溶液（pH 10．0）、体积分数为20％的丙酮作有机改性剂的条件下，可以在2．2 min内实现对嗪草酮、氰草津、西草津、莠去津、扑灭通、莠灭净、扑灭津、特丁净的分离检测。结合固相萃取（SPE）对农田水样进行测定，各农药检出限为0．5～2．5μg／L，回收率为88％～106％，相对标准偏差为3．3％～5．0％。A micellar electrokinetic chromatography method with lauric acid as a surfactant for rapid determination of triazine herbicides viz. metribuzin, cyanatryn, simetryn, atrazine, prometon, ametryn, propazine and terbutryn was developed. The effects of the concentration of lauric acid and tris-hydroxymethylaminomethane （Tris） in the buffer system, the pH of the buffer, the organic modifier, the sampling time, the capillary temperature and applied voltage on the separation of the triazine compounds were studied. Under optimized conditions （50 mmol/L lauric acid and 100 mmol/L Tris in buffer solution; 20% acetone as the organic modifiers, capillary temperature 25 ℃, applied voltage 25 kV, sampling time 6 s）, the baseline separation of eight triazine herbicides was achieved within 2. 2 min. The detection limit of the triazine compounds varied from 0. 5 μg/L to 2.5 μg/L （preconcentrated by ODS -C18 solid phase extraction） and the average recoveries ranged from 88% to 106% with RSDs below 5.0% （ n = 5）.

关 键 词：胶束电动毛细管电泳 月桂酸 三嗪类农药 水 固相萃取 

分 类 号：O657.8[理学—分析化学] S482.4[理学—化学]