鸡胚低分子多肽对D-半乳糖拟衰老小鼠学习记忆的影响  

EFFECTS OF LOW MOLECULAR WEIGHT POLYPEPTIDE EXTRACTED FROM CHICKEN EMBRYO ON LEARNING AND MEMORY IN AGING MICE INDUCED BY D-GALACTOSE

作　　者：陈姝[1] 何蕴韶[2] 程刚[2] 高劲松[2] 

机构地区：[1]广东省佛山市第一人民医院临床医学研究所,广东佛山528000 [2]中山大学达安基因诊断中心,广州510080

出　　处：《营养学报》2008年第1期95-97,共3页Acta Nutrimenta Sinica

基　　金：国家卫生部攻关计划项目(199983号)

摘　　要：目的探讨鸡胚低分子多肽对D-半乳糖(Gal)诱导的衰老小鼠学习记忆的影响。方法以孵化16d的鸡胚为原料经酸碱水解、反复酶解、超滤等过程制备鸡胚低分子多肽(LMP)。BALB/c小鼠48只,随机分为对照组、模型组、低分子多肽低剂量、和高剂量干预组,每组12只。模型组及两个干预组颈背部皮下注射80mg/(kgbw.d)Gal诱导衰老模型;对照组皮下注射8ml/(kgbw·d)生理盐水。前两组灌胃20ml/(kgbw·d)生理盐水;后两组分别灌胃10ml/(kgbw·d)和20ml/(kgbw·d)的鸡胚低分子多肽。采用Morris水迷宫观察小鼠学习记忆的行为学变化。结果模型组潜伏期比对照组明显延长(P<0.01),在平台象限游泳距离的百分比明显降低(P<0.01)。鸡胚LMP干预组与模型组比较,可明显缩短潜伏期(P<0.01),提高在平台象限游泳距离的百分比(P<0.01)。结论鸡胚低分子多肽有改善D-半乳糖拟衰老小鼠学习记忆的功效。Objective To investigate the effects of low molecular weight polypeptide（LMP） extracted from chicken embryo on learning and memory in senile mice induced by D-galactose（Gal）. Method The 16 d old chicken embryo was acid and alkali hydrolyzed, enzymolyzed and ultrafiltered to formulate LMP. 48 mice were divided randomly into four groups： control, aging model, aging＋low dose LMP, aging＋high dose LMP. The aging model and two LMP groups were treated with Gal 80 mg/ （kg bw · d） by nape subcutaneous injection, while the control group with normal saline 8ml/（kg bw · d）. Control and model groups were given i.g. normal saline 20 ml/（kg bw ·d）, and two LMP groups 10 ml or 20 ml/（kg bw ·d） LMP respectively. Learning and memory of mice were tested with Morris water maze. Results The escape latency of model group was longer and the percentage of swimming distance in platform region higher than that of control group （P〈0.01）. Compared with model group, the escape latency was shortened, and ratio of swimming distance in platform region was increased in LMP group （P〈0.01）. Conclusion The low molecular weight polypeptide extracted from chicken embryo can improve learning and memory of senile mice induced by D-galactose.[ACTA NUTRIMENTA SINICA, 2008,30（1）： 95- 97]

关 键 词：鸡胚 低分子多肽 D-半乳糖 衰老 学习记忆 

分 类 号：R151[医药卫生—营养与食品卫生学]