芦荟苦素对色素化皮肤类似物模型中黑素细胞的影响  

The effect of aloesin on melanocytes in the pigmented skin equivalent model

作　　者：杨壮群[1] 王正辉[1] 张铁良[2] 屠军波[1] 宋勇[1] 虎小毅[1] 李国光[1] 

机构地区：[1]西安交通大学口腔医院口腔颌面整形外科,710004 [2]宝鸡市口腔医院

出　　处：《中华整形外科杂志》2008年第1期50-53,共4页Chinese Journal of Plastic Surgery

基　　金：2004年陕西省自然科学基金资助项目（2004C229）

摘　　要：目的观察芦荟苦素、熊果苷及茶多酚对色素化皮肤类似物模型中黑素细胞的影响。方法体外构建色素化皮肤类似物模型，检测芦荟苦素、熊果苷及茶多酚作用于此模型后对细胞形态、黑素细胞酪氨酸酶活性以及黑素合成的影响。结果在色素化皮肤类似物模型中3种退色剂对黑素细胞酪氨酸酶活性有明显抑制作用，可显著减少黑素生成，其中以茶多酚的抑制作用最强，芦荟苦素次之。但茶多酚对细胞的毒性最大，熊果苷和芦荟苦素毒性均较小。结论3种退色剂对色素化皮肤类似物中黑素细胞的酪氨酸酶活性和黑素合成均呈浓度依赖性抑制，而茶多酚则显示出较大的细胞毒性（P〈0．05）。芦荟苦素所表现出的对酪氨酸酶和黑素的合成有较强的抑制作用及对细胞较低的毒性作用，有可能成为一种比较安全的退色剂。Objective To observe the effect of aloesin, tea polyphenols, arbutin on melanocytes in the pigmented skin equivalent model. Methods First, we constructed the pigmented skin equivalent model in vitro. And then we detected the effect of aloesin, tea polyphenols and arbutin on the cells＇ shape, tyrosinase activity and formation of melanin in the constructed pigmented skin equivalent, Results Three depigmenting agents showed an inhibition effect on the tyrosinase activity of melanocytes and reduced significantly melanin content in the pigmented skin equivalent model, in which the tea polyphenols had the strongest effect, and then was the aloesin. But the tea polyphenols showed the strongest toxicity, while the aloesin and arbutin had a much lower toxicity. Conclusions All the three depigmenting agents showed a concentration dependent suppression effect on the tyrosinase activity and formation of melanin, in which the tea polyphenols was the strongest effect（ P 〈 0.05）. Aloesin has a good suppression effect on the tyrosinase activity and formation of melanin, but has a much lower toxicity, which could be used as a safe depigmenting agent.

关 键 词：黑素细胞 芦荟苦素 色素化皮肤类似物模型 熊果苷 茶多酚 酪氨酸酶 退色剂 

分 类 号：R686[医药卫生—骨科学]