HPLC-ESI-MS^n法研究中乌头碱在水中的化学反应  

Study on chemical reaction of mesaconitine in water by HPLC-ESI-MS^n

作　　者：刘永刚[1] 于达亮[1] 陈玉娟[2] 张宏桂[1] 乔延江[1] 

机构地区：[1]北京中医药大学中药学院,北京100029 [2]吉林大学药学院,长春130021

出　　处：《中国新药杂志》2008年第2期153-156,共4页Chinese Journal of New Drugs

基　　金：国家重点基础研究发展规划项目课题(2004BA721A11)

摘　　要：目的:用高效液相色谱-电喷雾-质谱/质谱(HPLC-ESI-MSn)方法研究中乌头碱在水中发生的化学反应。方法:液相用梯度洗脱,质谱用正离子模式。结果:在水解液中发现了5种水解产物,其准分子离子[M+H]+分别为590,486,514,618,572。结论:通过对水解产物的准分子离子和碎片离子的初步分析,可以推测其水解产物为中乌头次碱、中乌头原碱和3个初步确定结构的化合物,并推测在水中可以发生脱乙酰基反应同时脱水的反应;脱N上的甲基或16位C上的甲基反应;脱苯甲酰基反应同时脱16位C上的甲基或脱N上的甲基。Objective：To study the hydrolysates of mesaconitine and deduce the chemistry of mesaconitine in water. Methods：The sample was analyzed with gradient elution by HPLC. A Agilent MS TRAP XCT/plus equipped with ESI source was used as detector and operated in positive ion mode. Results：Five hydrolysates were found. Their protonated molecularions at m/z 590,486,514,618,572 were monitored. Conclusion ： The hydrolysates were deduced to be benzoylmesaconine,mesaconine, and the other three were uncertain. The chemical reaction included hydrolysis, demethyl and debenzoyl reaction with different positions.

关 键 词：水解产物 中乌头碱 高效液相色谱-电喷雾-质谱/质谱 

分 类 号：R284.1[医药卫生—中药学]