戊四氮致痫大鼠对学习记忆及海马齿状回PSD-95表达的影响  

Effect of Pentylenetetrazol-kindled Epilepsy on Learning and Memory and Expression of Postsynaptic Density 95 in Rats

作　　者：王佩[1] 王冰[2] 刘瑞春[1] 王海祥[1] 王维平[1] 范月辉[1] 胡华伟[1] 

机构地区：[1]河北医科大学第二医院神经内科,河北省石家庄市050000 [2]河北医科大学第三医院神经内科

出　　处：《中国全科医学》2008年第3期218-219,共2页Chinese General Practice

基　　金：河北省自然科学基金资助项目(C2007000852);河北省科技攻关项目(NO:07276101D-21)

摘　　要：目的探讨戊四氮(PTZ)点燃癫痫对大鼠空间学习记忆及海马齿状回突触后致密物-95(PSD-95)表达的影响。方法戊四氮腹腔注射点燃慢性癫痫(CEP)模型,采用Morris水迷宫对大鼠进行行为学检测,运用免疫组织化学方法观察海马齿状回PSD-95的表达。结果癫痫组大鼠空间学习记忆能力受损;其海马齿状回PSD-95免疫阳性产物与对照组间差异有统计学意义(P<0.01)。结论戊四氮点燃癫痫大鼠空间学习记忆受损可能与海马神经元PSD-95的表达减少有关。Objective To explore the effect of epilepsy kindled by pentylenetetrazol （PTZ） on learning and memory and expression of postsynaptic density 95 （PSD - 95） in rats. Methods Rats were given PTZ till being kindled successfully. Their behaviors were afterwards determined by Morris water maze and the expression of PSD -95 in hippocampus dentate gyrus （DG） by immunohistochemical method. Results The ability of learning and memory was impaired and the immunoreactivity of PSD - 95 decreased simultaneously in rats with pentylenetetrazol - kindled epilepsy （ P 〈0. 01 ）. Conclusion The impairment of the ability of learning and memory might be related with the change of PSD - 95 in hippocampal neurons in rats with pentylenetetrazol - kindled epilepsy.

关 键 词：戊四氮 癫痫 神经行为学表现 突触后致密物95 

分 类 号：R742.1[医药卫生—神经病学与精神病学]