实时荧光定量RT-PCR法测定脑胶质瘤中MT1-MMP基因mRNA表达水平  

mRNA expression of MT1-MMP gene in glioma by quantitative real-time fluorescence RT-PCR

作　　者：刘维生[1] 耿会娟[2] 王硕[1] 肖新如[1] 陈善文[1] 赵继宗[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科,北京100050 [2]首都医科大学附属北京天坛医院检验科,北京100050

出　　处：《中国微侵袭神经外科杂志》2008年第2期75-77,共3页Chinese Journal of Minimally Invasive Neurosurgery

摘　　要：目的探讨胶质瘤恶性程度和MT1-MMP基因mRNA表达水平的关系。方法采用实时荧光定量RT-PCR法检测34例脑胶质瘤和7例正常脑组织(取自癫疒间病人手术切除脑组织)中MT1-MMP的mRNA表达水平。结果经实时荧光定量RT-PCR法检测,正常脑组织和低级别、高级别胶质瘤的△△Ct(循环阈值)值分别为(0.834±0.517)、(4.063±2.309)和(7.072±4.072)。正常脑组织与低级别、高级别胶质瘤之间,低级别、高级别胶质瘤之间MT1-MMP基因mRNA表达水平的差异具有统计学意义(P<0.05)。结论脑胶质瘤中存在MT1-MMP基因mRNA高水平的表达,随着肿瘤恶性级别的增加,MT1-MMP基因mRNA表达水平有增高趋势。Objective To explore the relationship between malignancy of glioma and mRNA expression of MT1-MMP gene. Methods The mRNA expression of MT1-MMP gene in 34 gliomas and 7 normal brain tissues （from epileptic focus resection） was detected by quantitative real-time RT-PCR method. Results By Real-time RT-PCR method, the △△Ct（cycle threshold） values of the normal brain tissue and low-grade, high-grade glioma were 0.834±0.517, 4.063±2.309, and 7.072±4,072 respectively. There was statistical significance of difference between normal brain tissue and low-grade, high-grade glioma, and between low-grade and high-grade glioma （P〈0.05）. Conclusion The mRNA expression of MT1-MMP gene is up-regulated in glioma, and increases with the malignancy grade of glioma.

关 键 词：神经胶质瘤 间质胶原酶 逆转录聚合酶链式反应 

分 类 号：R739.4[医药卫生—肿瘤]