胃泌素受体拮抗剂丙谷胺对原代培养大肠癌细胞作用的研究  

Effects of gastrin receptor antagonist proglumide on primary large intestinal cancer cell culture

作　　者：何双梧[1] 谢尚奎 赵言明[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所普外科,重庆630042 [2]157医院普普外科,广州510510

出　　处：《中华消化杂志》1997年第2期91-93,共3页Chinese Journal of Digestion

摘　　要：目的：在体外观察胃泌素受体持抗剂丙谷胺（PGL）对原代培养大肠癌活细胞数（吸光度，A值）和DNA合成（脉冲数，CPM值）的影响，为大肠癌病人临床应用胃泌素受体拮抗剂治疗提供实验依据。方法：对25例大肠癌根治术病人的新鲜标本进行细胞分离和原代培养，采用MTT比色分析法检测活细胞数；3H－TdR掺入法测定DNA合成．结果：PGL组高、中和低分化腺癌活细胞数和DNA合成与对照组比差异均无显著性（P均>0.05),5肽胃泌素（PG）组活细胞数和DNA合成均显著高于对照组（P均＜0．01），PGL＋PG组活细胞数和DNA合成均显著低于PG组（P均＜0．01），而与对照组比差异无显著性（P均＞0．05）。结论：丙谷胺对原代培养大肠癌细胞的增殖无明显影响，但可抑制胃泌素对大肠癌细胞的促增殖作用。Objectives: In order to analyze the effects of gastrin receptor antagonist proglumide(PGL) on cell viability (A value) and DNA synthesis (CPM value) of the primary large intestinalcarcinoma cell culture. Methods: The primary large intestinal carcinoma cell culture was obtained from 25 patients. Cell viability (A value) DNA synthesis (CPM value) and were measured in vitro using MTT assay and 3H－TdR incorporation. Results: In PGL group, the cell viability and CPM value in well, medium and poorly differentiated carcinoma were not significantly different as compared with control group (P＞0.05). In pentagastrin(PG) group, the A and CPM values were higher than those of controls (P＜0.01), whereas in PG plus PGL group, the A and CPM values were lower than those of PG group (P＜0.01), but the difference was not significant as compared with controls (P＞0.05). Conclusions: It is suggested that PGL had no significant proliferative effect on primary large intestinal carcinoma cell culture. However, it may inhibit the proliferative effect of PG on large intestinal carcinoma cells. These results provide a therapeutic basis of using gastrin receptor antagonist for patients with large intestinal carcinoma.

关 键 词：大肠肿瘤 丙谷胺 胃泌素 

分 类 号：R735.340.2[医药卫生—肿瘤]