中华眼镜蛇毒对K562和难治性白血病细胞体外作用的研究  

Effect of Naja Naja Actra Venom on K562 and refractory leukemia cells

作　　者：吴穗晶[1] 杜欣[1] 蒋文玲[1] 张建军[1] 周茂华[1] 黄志新[1] 耿素霞[1] 林伟[1] 黄梓伦[1] 

机构地区：[1]广东省人民医院,广东广州510080

出　　处：《现代肿瘤医学》2008年第3期428-430,共3页Journal of Modern Oncology

基　　金：广东省中医药局资助项目(编号:A1040114)

摘　　要：目的:探讨中华眼镜蛇毒(Naja Naja Actra Venom,NNAV)抗白血病作用。方法:应用不同剂量NNAV和阿霉素(THP)于人红白血病细胞株(K562)和难治性白血病细胞株,观察NNAV直接或协同THP的细胞毒性及采用流式细胞仪进行凋亡检测。结果:NNAV对K562有明显的细胞毒及促进细胞凋亡作用,并剂量依赖地增强THP杀伤作用。但对难治性白血病细胞株作用不一:单用NNAV有细胞毒性25%(5/20);单用无效联合THP有效55%(11/20),以NNAV 100μg/ml+THP 5μg/ml作用最强;凋亡百分率增多60%(12/20)。临床对比研究结果,体外实验THP及NNAV无效的9例病人,含蒽环类全部无效(其他机制药物有效3例);THP及NNAV有效的5名病人,含蒽环类缓解2例,1例其它药物缓解;双相吻合率超过60%。结论:NNAV体外对难治性白血病细胞株有一定杀伤作用,和适当剂量THP合用有效率及杀伤作用明显增加,提示蛇毒中可能含有特殊抗难治性白血病的组分。Objective：To investigate the effect of Naja Naja Actra Venom （NNAV） on leukemia cells. Methods： To detect cytotoxic effect of NNAV and THP at different dose level on K562 and refractory leukemia cells with MTr and FCM. Results： NNAV showed inhibitory effect on K562, this influence could be strengthened with THP in a dose - dependent manner. But the relations changed in refractory leukemia cells： 25 % （5/20） of samples were sensitive to single NNAV , 55% （11/20） were sensitive to combination of NNAV and THP, the strongest effect was produced by 100p, find of NNAV and 5μg/nd of THP. Percentage of apotosis in 60% （12/20）of samples raised up. Furthermore, clinical treatment with anthracyclines also failed in 9 patients who were not sensitive to NNAV and THP in vitro, but 3 of these reached release with other chemical drugs, 2 patients received release with anthracyclines and 1 patient reached release with other drugs in 5 patients who were sensitive to NNAV and THP in vitro. Conclusion： NNAV has cytotoxic effect on refractory leukemia cells in vitro , which is more stronger with approciate dose of THP. It indicates that there is some special fraction in NNAV showed anti - refractory leukemia cells effect.

关 键 词：中华眼镜蛇毒 难治性白血病 人红白血病细胞株 

分 类 号：R733.7[医药卫生—肿瘤]