Phytosterol of Potamogeton maackianus and Its Change under the Stress of Microcystis aeruginosa  

作　　者：ZHANG Shenghua WU Zhenbin CHENG Shuiping LIANG Wei WU Xiaohui 

机构地区：[1]Institute of Hydrobiology, Chinese Academy of Sciences / State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan 430072, Hubei, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Wuhan University Journal of Natural Sciences》2008年第1期113-117,共5页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Science Fund for Distinguished Young Scholars (39925007);the National High Technology Research and Development Program of China (863 Program) (2002AA601021)

摘　　要：The phytosterol in Potamogeton maackianus was identified and quantified. From the hexane extracts analysis of unsaponifiable fraction ofP. maackianus by gas chromatography - mass spectrometry（GC-MS）, campesterol, stigmasterol and β-sitosterol were the main phytosterols in P. maackianus with the contents of 0.368, 1.17 and 0.824 mg·g^1, respectively. After 7 days＇ exposure under M. aeruginosa, the contents of campesterol, stigmasterol and β-sitosterol in P.maackianus increased by 23.1%, 40.4% and 40.8%, respectively. Both the contents of 24-ethyl sterols （sitosterol and stigmasterol） and 24-methyl sterol （campesterol） in macrophytes increased, but the percentage of total sterol composition did not change significantly. This result indicated that the content of 4-demethyl sterols and the second alkylation of the phytosterol side-chain at C-24 in P. maackianus were affected by M, aeruginosa.The phytosterol in Potamogeton maackianus was identified and quantified. From the hexane extracts analysis of unsaponifiable fraction ofP. maackianus by gas chromatography - mass spectrometry（GC-MS）, campesterol, stigmasterol and β-sitosterol were the main phytosterols in P. maackianus with the contents of 0.368, 1.17 and 0.824 mg·g^1, respectively. After 7 days＇ exposure under M. aeruginosa, the contents of campesterol, stigmasterol and β-sitosterol in P.maackianus increased by 23.1%, 40.4% and 40.8%, respectively. Both the contents of 24-ethyl sterols （sitosterol and stigmasterol） and 24-methyl sterol （campesterol） in macrophytes increased, but the percentage of total sterol composition did not change significantly. This result indicated that the content of 4-demethyl sterols and the second alkylation of the phytosterol side-chain at C-24 in P. maackianus were affected by M, aeruginosa.

关 键 词：Potamogeton maackianus Microcystis aeruginosa PHYTOSTEROL enviromental stress 

分 类 号：O65[理学—分析化学]