甲状腺相关眼病患者与正常人眼眶前脂肪细胞表面抗原表达的比较  

Cell surface antigen expression of orbital preadipocytes between patients with thyroid-associated ophthalmopathy and healthy adults

作　　者：雷翔[1] 许雪亮[1] 

机构地区：[1]中南大学湘雅医院眼科,长沙410008

出　　处：《中南大学学报（医学版）》2008年第1期43-46,共4页Journal of Central South University ：Medical Science

基　　金：湖南省科技厅重点资助项目(05jj-148)~~

摘　　要：目的:研究甲状腺相关眼病(TAO)与正常人眼眶前脂肪细胞表面抗原的表达。方法:采用组织块培养法对TAO和正常人的眼眶前脂肪细胞进行体外培养,用流式细胞术对其细胞表面抗原CD29,CD44,CD49d,HLA-DR进行检测。结果:TAO和正常人眼眶前脂肪细胞均表达CD29,CD44和CD49d,TAO患者较正常人表达HLA-DR明显增强(P<0.05)。结论:TAO患者与正常人的眼眶前脂肪细胞均来源于间充质干细胞,TAO的眼眶前脂肪细胞可能参与了TAO的眶内炎症。Objective To study the cell surface antigen expression of orbital preadipocytes between patients with thyroid-associated ophthalmopathy （TAO） and healthy adults. Methods Tissue culture methods were used in the cell culture of orbital preadipocytes in patients with TAO and healthy adults. Flow cytometry was used to analyze the expression of CD29, CD44, CD49d, and HLA-DR in these 2 cells. Results Positive expressions were examined in orbital preadipocytes of patients with TAO and healthy adults. The orbital preadipocytes of patients with TAO had a higher expression of HLA-DR than that of healthy adults. Conclusion The orbital preadipocytes of patients with TAO and healthy adults cultured by tissue cultured methods both belong to mesenchymal stem cells, and the former may participate in the orbital inflammation.

关 键 词：甲状腺相关眼病 眼眶 前脂肪细胞 细胞表面抗原 

分 类 号：R771.3[医药卫生—眼科]