高效液相色谱法测定复方益母胶囊中阿魏酸的含量  

Content Determination of Ferulic Acid in Compound Prescribe Motherwort Herb Capusle by HPLC

作　　者：张国民 齐赤虹 杨工昶 李志军[3] 

机构地区：[1]开封市食品药品检验所,河南开封475002 [2]河南省医药学校,河南开封475001 [3]河南大学药学院,河南开封475004

出　　处：《中国中医药信息杂志》2008年第2期48-50,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立高效液相色谱法测定复方益母胶囊中阿魏酸含量的方法。方法采用ZORBAXSB-C18色谱柱,流动相为乙腈-0.2%磷酸水溶液(12︰88),检测波长为316nm,流速为1.0mL/min。结果阿魏酸在0.8～13μg/mL范围内呈良好的线性关系(r=0.99999,n=6),平均回收率为103.0%(RSD=0.4%,n=5)。结论本法简单可行,准确度高,重现性好,回收率高,能有效控制复方益母胶囊的质量。Objective To estblish a HPLC method for content determination of ferulic acid in compound prescribe Motherwort Herb capusle. Method The column ZORBAXSB-C18 was used with Acetonitrile-0.2% phosphoric acid aqueous solution （12 ： 88） as mobile phase, and detective wavelength was 274 nm, the flow rate was 1.0 mL/min. Result Within the range of 0.8-13 μg, ferulic acid presented a fine linear relationship （r=0.999 99, n=6）. The average recovery was 103.0% （RSD=0.4%, n =5）. Conclusion The method is suitable, accuracy is high and reproducibility is good, recovery is high, and can control the quality of compound prescribe Motherwort Herb capusle.

关 键 词：复方益母胶囊 阿魏酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]