用酵母双杂交系统筛选与拟南芥成膜素相关蛋白(PhrIP1)相互作用的蛋白质  

Identification of Protein Interaction with Phragmoplastin-interacting Protein1 (PhrIP1) by Yeast Two-hybrird System

作　　者：马立安[1] 王芳[2] 张忠明[3] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]华中农业大学作物遗传改良国家重点实验室,湖北武汉430070 [3]华中农业大学农业微生物学国家重点实验室,湖北武汉430070

出　　处：《长江大学学报（自科版）（中旬）》2007年第4期58-61,共4页Journal of Yangtze University(Nature Science Edition)

基　　金：国家自然科学基金项目(30070370);美国国家科学基金项目(NSF-MCB0548525)

摘　　要：用酵母双杂交系统,以pEG202-PhrIP1为诱饵筛选拟南芥AD-cDNA文库,寻找与成膜素相关蛋白(PhrIP1)相互作用的蛋白质。结果表明,在5×106个转化子中得到6个阳性克隆,经测序及体外蛋白质相互作用证实,得到与PhrIP1相互作用的2个靶蛋白,即Ran2小GTP结合蛋白和CIPK6钙调节激酶。To identify proteins that interact with PhrIP1,the yeast two-hybrid system was taken to screen AD-cDNA library of Arabidopsis thaliana by using PhrIP1(pEG202-PhrIP1) as bait. The results showed that 6 genes were isolated from the library from a yield of 5×106 transformants and two of them encoding proteins could be interacted with PhrIP1 in a protein-protein binding assay in vitro,and Ran2 small GTP binding protein and CBL-interacting protein kinase 6 (CIPK6) by sequencing and blast are determined as the target proteins to study.

关 键 词：酵母双杂交系统 成膜素相关蛋白(PhrIP1) Ran2小GTP结合蛋白 CIPK6钙调节激酶 

分 类 号：Q785[生物学—分子生物学]