硫酸镁调节SO2衍生物对大鼠海马神经元钠通道的刺激作用  

Stimulation Effect of SO_2 Derivatives Modulated by MgSO_4 on Sodium Channels in Rat Hippocampal Neurons

作　　者：桑楠[1] 云洋[1] 李广科[1] 孟紫强[1] CHEN Chu 

机构地区：[1]山西大学环境科学与工程中心,环境与资源学院,太原030006 [2]Neuroscience Center of Excellence, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

出　　处：《应用与环境生物学报》2008年第1期74-77,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No20607013);山西省青年科学基金项目(No20051043)资助~~

摘　　要：利用全细胞膜片钳技术研究了硫酸镁(MgSO4)对SO2衍生物刺激大鼠海马神经元电压门控性钠通道的调节作用.结果表明,MgSO4对SO2衍生物增大大鼠海马神经元钠电流的效应具有抑制作用,此作用随着SO2衍生物浓度的增加而减弱,随着MgSO4浓度的增加而增强.不同时间加入MgSO4均会显著抑制SO2衍生物增大钠电流的效应,说明MgSO4不仅对SO2衍生物增大钠电流的效应具有抑制作用,而且可即时和延效性地抑制此损伤效应的发生.研究结果提示,硫酸镁对SO2衍生物刺激大鼠海马神经元钠通道具有抑制作用,这可能是MgSO4对SO2引发的中枢神经系统损伤具有防护和治疗作用的机制之一.Modulation of MgSO4 for SO2 derivative - induced stimulation on voltage - gated sodium channels in rat hippocampal neurons was investigated by using the whole-cell patch clamp techniques. The results indicated that MgSO4 attenuated SO2 derivative-induced augment on Na^＋ currents, and this action was positively related with the concentration of MgSO4 but negatively related with the concentration of SO2 derivatives. In addition, adding MgSO4 at different times would obviously inhibit the effect of SO2 derivatives on Na^＋ currents. It suggested that MgSO4 inhibited SO2 derivative-induced stimulation on voltage-gated sodium channels in rat hippocampal neurons, which implicates the possible mechanisms for MgSO4 protecting neuron against neuronal dysfunction resulting from SO2 exposure. Fig 4, Ref 14

关 键 词：海马神经元 全细胞膜片钳技术 MgSO4 SO2衍生物 电压门控性钠通道 

分 类 号：X174[环境科学与工程—环境科学]