土壤重金属复合污染对脲酶、磷酸酶及脱氢酶的影响  

Kinetics characters of soil urease, acid phosphotase and dehydrogenase activities in soil contaminated with mixed heavy metals

作　　者：滕应[1] 骆永明[1] 李振高[1] 

机构地区：[1]中国科学院南京土壤研究所,土壤与农业可持续发展国家重点实验室,江苏南京210008

出　　处：《中国环境科学》2008年第2期147-152,共6页China Environmental Science

基　　金：国家自然科学基金资助项目(40432005);国家“973”项目(2002CB410809/10);中国科学院知识创新项目(kzcx2-yw-404、CXTD-Z2005-4)

摘　　要：采用模拟实验对铅锌矿区重金属(Pb、Zn、Cu、Cd)复合污染土壤酶活性变化的动力学参数进行了研究.结果表明,矿区土壤酶活性随着重金属污染程度的加剧而显著降低.尾矿区土壤脲酶、酸性磷酸酶、脱氢酶的Vmax平均值分别是非矿区土壤的60%、77%、38%,而土壤脲酶和酸性磷酸酶的Km平均值分别是对照土壤的2.73和2.25倍.逐步回归和通径分析结果表明,矿区土壤中Pb、Zn、Cu、Cd元素含量与脲酶、磷酸酶及脱氢酶Vmax和Km之间均存在着显著或极显著的相关关系;4种重金属元素对不同性质酶酶动力学参数的相对贡献大小存在差异,且元素之间也存在着交互效应.The kinetics characters of enzymatic activities in soil contaminated with heavy metals in the lead-zinc tailing mine were studied. Soil enzyme activities decreased markedly with increasing degree of soil pollution caused by heavy metals in the mine area. The average Vmax values of soil urease, phosphotase and dehydrogenase in the mine area were 60%,77% and 38% of those of non-mine area, and that the average Km values of soil urease, phosphotase were 2.73 and 2.25 times than those of non-mine area, respectively. The stepwise regression and path analysis further showed that kinetics parameters （Vmax and Km） of the reaction promoted by soil urease, phosphotase and dehydrogenase were significantly correlated with the concentrations of heavy metals such as Pb, Zn, Cu and Cd in soil of the mine area. However, the degree of the influence of each heavy metal on the activity of different enzymes was greatly different, and moreover, there was an interacting effect of four heavy metals.

关 键 词：重金属复合污染 土壤酶活性 动力学参数 

分 类 号：X131.3[环境科学与工程—环境科学] S154.36[农业科学—土壤学]