The Immuno-fluorescence Quantity Analysis of α-Tubulin and γ-Tubulin Protein in Precancerous Lesion and Carcinoma of the Breast and Its Significance  

乳腺癌前病变和乳腺癌中α、γ-tubulin蛋白表达的免疫荧光定量分析及其意义(英文)

作　　者：牛昀[1] 张彤文 王颖 韦丽[1] 魏锡胤 牛瑞芳[1] 

机构地区：[1]天津医科大学附属肿瘤医院(国家)教育部乳腺癌防治重点实验室 [2]Medical Colege,Nankai University

出　　处：《Chinese Journal of Clinical Oncology》2008年第1期16-21,共6页中国肿瘤临床（英文版）

基　　金：国家自然科学基金项目资助[项目编号:30471967]~~

摘　　要：OBJECTIVE To investigate the changes and values of the expression of α-tubulin and γ-tubulin in atypical ductal hyperplasia （ADH）, ductal carcinoma in situ （DCIS） and invasive ductal carcinoma （IDC） of the breast. The relationship between centrosome abnormalities and breast tumor development was further discussed. METHODS There were three groups including ADH, DCIS and IDC with 30 cases in each group. They were analyzed by immuno-fiuorescence quantity analysis. The expression levels of α-tubulin and γ-tubulin protein in these tissues were detected by flow cytometry immuno-fiuorescence analysis and compared with the results from normal tissues. Immunohistochemistry was also performed in this research. RESULTS The results showed significant differences of the average of the positive （FITC labeled） cells （P=0.000） among the four groups. The level of the IDC group was the highest, while normal breast tissue showed the lowest level. The results suggested that the expression levels of α-tubulin and γ-tubulin both increased as the grade of cellular proliferation and differentiation increased. The expressions showed significant differences among all the groups, except between the ADH and DCIS. There were no significant differences between α-tubulin and γ-tubulin expression in each group （P〈0.05）, as there was agreement in the immuno-fluorescence and immunohistochemical analysis for protein expression. CONCLUSION There is abnormal expression of centrosome tubulin as an early event in the development of breast tumor. Furthermore these aberrations may play a key role during oncogenesis and promote cellular transformation to malignancy. The immuno-fiuorescence quantitive analysis and immunohistochemistry can complement each other.目的:研究乳腺导管上皮不典型增生(ADH)、导管内癌(DCIS)及浸润性导管癌(IDC)中α-tubulin和Γ-tubulin表达的变化及其意义,进一步探讨中心体异常与乳腺癌发生发展的相关性。方法:研究样本被分为导管上皮不典型增生、导管内癌和浸润性导管癌3组,每组30例。采用流式细胞免疫荧光定量分析技术和免疫组织化学方法,检测上述各组中心体微管蛋白α-tubulin和Γ-tubulin表达水平,与30例正常乳腺组的检测结果比较,并进行统计学分析。结果:在全部样本组中α-tubulin及Γ-tubulin FITC标记的阳性细胞数均数不同,差异有显著性(P=0.000),浸润性导管癌组的过表达最高,正常组织组最低。α-tubulin、Γ-tubulin表达水平随着细胞增殖级别的增加和分化程度的变差呈增高趋势。除不典型增生组和导管内癌组两组间比较以外,其余各组间差异均有显著性(P=0.001)。在每组中α-tubulin和Γ-tubulin的表达程度无统计学差异(P<0.05),显示免疫荧光定量分析和免疫组织化学检测结果趋于一致。结论:乳腺癌发生的早期阶段即存在着中心体蛋白的过度表达,推测这种异常表达在促进细胞恶性转化、形成乳腺癌过程中可能扮演了重要角色。对于中心体蛋白过表达的检测,免疫荧光定量和免疫组化两种技术可相互补充。

关 键 词：centrosome α-tubulin protein centrosome γ-tubulin protein precancerous lesion breast carcinoma immuno-fluorescence quantity analysis. 

分 类 号：R737.9[医药卫生—肿瘤]