不同取卵时间对兔ICSI胚胎体外发育的影响  

Effects of Time of Oocyte Collection after Superovulation on in vitro Development of Intracytoplasmic Sperm Injection Rabbit Embryos

作　　者：陈浩杰[1] 刘丽均[2] 李晟阳[1] 赵立虎[1] 徐平[2] 罗光彬[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]中国科学院上海实验动物中心,上海201615

出　　处：《中国比较医学杂志》2008年第2期32-34,共3页Chinese Journal of Comparative Medicine

基　　金：国家自然科技资源条件平台项目(2004DKA30650);上海市科技发展基金(0549090007)

摘　　要：目的探讨不同取卵时间对兔ICSI胚胎体外发育的影响。方法采用Piezo操作系统对实验兔进行辅助体外受精。结果hCG注射后14、161、8 h取卵,ICSI后的受精率分别为82.2%、75.9%和0.0%,对受精卵进行体外发育培养,桑椹胚的发育率分别为72.9%、70.0%、0.0%,囊胚的发育率分别为62.2%、53.3%、0.0%。14 h和16 h之间受精率、桑椹胚率、囊胚率差异不显著(p>0.05),但是14 h采卵比16 h要好;18 h和14 h1、6 h之间差异显著(P<0.05)。结论不同取卵时间影响实验兔的ICSI体外受精率及胚胎的体外发育率,hCG注射后14 h取卵最有利于兔ICSI胚胎的发育。Objective This paper described the use of piezo-driven micropipette for intracytoplasmic sperm injection （ICSI） of rabbit eggs and discussed the effects of time of oocyte collection after superovulation on in vitro development of intracytoplasmic sperm injection rabbit embryos. Results Oocytes were collected at 14 h, 16 h, 18 h after hCG administration. The ICSI fertilization rate were 82.2% , 75.9% and 0.0%, respectively. The fertilized oocytes were further cultured in vitro. The morulae rates were 72.9% , 70.0% and 0.0%, and the rates of blastocyst were 62.2%, 53.3% and 0.0%, respectively. The rate of fertilization, morulae rate and the rate of blastocyst were not significantly different （ P 〉 0.05） between 14 h and 16 h groups, but were significantly different （ P 〈 0.05 ） between 14 h, 16 h and 18 h groups, but oocytes collected at 14 h after hCG administration were better than that of 16 h after hCG treatment. Conclusion Oocytes collected at different time after hCG injection has different effect on the fertilization rate of oocytes and development rate of intracytoplasmic sperm injection rabbit embryos. Oocytes collected at 14 h after hCG injection are most beneficial to the development of intracytoplasmic sperm injection rabbit embryos.

关 键 词：兔 单精注射 受精率 胚胎发育率 

分 类 号：R-33[医药卫生]