载脂蛋白E基因多态性与动脉硬化性脑梗死的相关研究  

Correlation research on apolipoprotein E gene polymorphism and atherosclerotic cerebral infarction

作　　者：王小娟[1] 徐恩[1] 官国东[1] 

机构地区：[1]广州医学院第二附属医院神经内科,广州510260

出　　处：《中华神经医学杂志》2008年第2期163-166,共4页Chinese Journal of Neuromedicine

基　　金：广东省自然科学基金（31776）

摘　　要：目的研究载脂蛋白E（ApoE）基因多态性与血脂水平、颈动脉斑块以及动脉硬化性脑梗死（CD的关系。方法对166名CI患者及72名健康成人采用聚合酶链反应-限制性片段长度多态性方法检测ApoE基因多态性，酶法检测血脂，颈动脉超声多普勒检查颈动脉斑块。结果CI组ε2／4＋ε3／4＋ε4/4型分别与ε2／2＋ε2／3型、ε3/3型相比，血浆总胆固醇（TC）显著升高（P〈0．05）。低密度脂蛋白胆固醇（LOL-C）显著升高（P〈0．05）；CI组ε2/4＋ε3／4＋ε4／4型与ε3/3型相比，患者颈动脉斑块较大（P〈0．05）；CI组ε4等位基因频率显著高于对照组（P〈0．05）。结论ApoEε4可能与血浆TC、LDL-C升高相关。推测是脑梗死的易感基因。Objective To investigate the relationship between apolipoprotein E （ApoE） gene polymorphism and atherosclerotic cerebral infarction （CI）, and explore the effect of ApoE gene polymorphism on the level of plasma lipids and carotid atherosclerotic plaques in patients with atherosclerotic CI. Methods ApoE gene polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）, the level of plasma lipids was measured by a standard enzymatic method and carotid plaques were detected by color Doppler ultrasonography in 166 patients with atherosclerotic CI （CI group） and 72 healthy adults （control group）. Results Compared with ε2/2 ＋ε2/3 and ε3/3 carders, ε2/4 ＋ε3/4 ＋ε4/4 carriers had significantly higher plasma total cholesterol （TC） levels （P〈0.05） and low-density lipoprotein cholesterol （LDL-C） levels （P〈0.05） in the CI group; ε2/4 ＋ε3/4 ＋ε4/4 carders had larger carotid plaques than ε3/3 carders in the CI group （P〈0.05）; the ε4 allele frequency in the CI group was significantly higher than that in the control group （P〈0.05）. Conclusion ApoE ε4 might be associated with the increase in plasma TC levels and LDL-C levels. ApoE ε4 might be a susceptible genetype of CI.

关 键 词：载脂蛋白E 基因多态性 动脉粥样硬化 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]