5-羟色胺在豚鼠离焦性近视眼中含量的变化  

The change of 5-hydroxytryptamine in guinea pigs with lens-induced defocus myopia

作　　者：杨积文[1] 徐艳春[1] 孙林[2] 田小丹[1] 

机构地区：[1]中国医科大学附属第一医院眼科,中国辽宁省沈阳市110001 [2]中国医科大学实验技术中心,中国辽宁省沈阳市110001

出　　处：《国际眼科杂志》2008年第2期288-290,共3页International Eye Science

摘　　要：目的:探讨5-羟色胺(5-hydroxytryptamine,5-HT)在豚鼠近视离焦模型形成中的作用。方法:将24只4周龄的豚鼠分成2组,离焦组(18只36眼),包括离焦实验眼(右眼,戴接触镜)和离焦对照眼(左眼,未戴接触镜);空白对照组(6只12眼)除检查外不做任何干预。将PMMA接触镜配戴在离焦组豚鼠右眼前,自然光下饲养14d后除去镜片,建立近视离焦模型。近视离焦模型建立后,分别处死实验组及空白对照组豚鼠,取出眼球,分离出视网膜、脉络膜及巩膜组织。用高效液像色谱电化学检测法测定视网膜、脉络膜及巩膜组织中5-HT含量。结果:在豚鼠视网膜、脉络膜、巩膜内均有5-HT存在,其中脉络膜中含量最高,其次为巩膜及视网膜;离焦实验眼视网膜、脉络膜及巩膜中5-HT含量明显升高(P<0.01);与离焦对照眼及空白对照组相比较差异显著(P<0.05)。结论:5-HT可能参与了离焦性近视的形成。AIM： To investigate the role of 5-hydroxytryptamine （5- HT ） in guinea pigs with lens-induced defocus myopia. METHODS： Twenty-four four-week-old healthy guinea pigs were randomly divided into wo groups： defocus group （18 guinea pigs, 36 eyes） including defocus experimental eyes （18 right eyes, wearing contact lens） and defocus control eyes （18 left eyes, not wearing contact lens）; blank control group （6 guinea pigs, 12 eyes） which underwent no intervention except for examinations. Riht eyes of guinea pigs in defocus group wore -10.00D PMMA contact lens and they were reared in natural light for 14 days. Then contact lenses were removed to set up models of defocus myopia. Subsequently all guinea pigs were executed and eyeballs were obtained to separate the retina, choroid and sclera. The contents of 5-HT in 36 eyes of defocus group and 12 eyes of control group were determined by high-performance liquid chromatography with electrochemical detection. RESULTS： There was 5-HT in the retina, choroid and sclera of guinea pigs, and the content of 5-HT was most in the choroid. The contents of 5-HT in the retina, choroid and sclera of defocus experimental eyes with lens-induced myopia were remarkably elevated （ P〈 0.01 ）. There were statistically significant differences compared with defocus control eyes and blank control group （ P〈 0.05）. CONCLUSION： 5-HT may play an important role in the formation of myopia.

关 键 词：离焦性近视 5-羟色胺 豚鼠 高效液像色谱/电化学检测法 

分 类 号：R778.11[医药卫生—眼科]