阳离子脂质体介导反义寡核苷酸肿瘤治疗的初步探索  

Study on Antitumor Effect of Antisense Oligodeoxynucleotide Against Telomerase By Cationic Liposome

作　　者：崔山风[1] 夏晓静[1] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100

出　　处：《中国药师》2008年第2期176-177,共2页China Pharmacist

摘　　要：目的:探讨阳离子脂质体(CL)介导反义寡核苷酸(ASODN)肿瘤治疗的效果。方法:以CL/ASODN复合物处理7721肝癌细胞,观察肝癌细胞的生长增殖和凋亡情况以及在体荷瘤小鼠治疗情况的考察。结果:CL/ASODN对7721肝癌细胞的抑制作用具有剂量和时间依赖性;荷瘤小鼠的实体瘤体积明显减小。结论:阳离子脂质体(CL)有望成为肿瘤治疗的理想载体。Objective： To investigate the antitumor effect of antisense oligodeoxynucleotide against telomerase by Cationic liposome. Method： The effect of inhibition with CL/ASODN complexes on proliferation of 7721 hepatocarcinoma cells was examined with MTF assay. The animal model was performed by direct intratumoral injection to mice bearing B16 cells. Result： The experiment indicated that the proliferation of 7721 hepatocarcinoma ceils could be inhibited using CL/ASODN complexes, and the inhibition profiles were in time and dose dependent manner. The volume of tumor treated with CL/ASODN obviously decreased compared to the control group after 6 days treatment. Conclusion： The experiment showed CL is a promising formulation for tumor therapy.

关 键 词：阳离子脂质体 反义寡核苷酸 MTF法 荷瘤小鼠 肿瘤治疗 

分 类 号：R965.1[医药卫生—药理学]