喉癌组织中MIEN1和HSP90基因表达的研究  

MKRN1 and HSP90 Expression in Laryngeal Squamous Cell Carcinomas

作　　者：王淑云[1] 李福才[1] 李婵媛[1] 郭星[2] 李晓渝[2] 

机构地区：[1]中国医科大学基础医学院医学遗传学教研室,沈阳110001 [2]中国医科大学附属第一临床医学院耳鼻喉科,沈阳 110001

出　　处：《国际遗传学杂志》2008年第1期1-4,共4页International Journal of Genetics

基　　金：辽宁省自然科学基金（2001101039）

摘　　要：目的正常细胞中MKRN1和HSP90基因的平衡决定了端粒酶活性和端粒长度，使细胞保持正常的状态；二者的表达异常可能与肿瘤的发生发展有关。为探讨MKRN1和HSP90基因的表达与喉癌发生发展之间的关系，我们研究了喉癌组织中MKRN1和HSP90基因mRNA的表达情况。方法提取50例喉癌组织的总mRNA，用半定量RT—PCR的方法检测MKRN1和HSP90基因mRNA的表达情况。结果50例喉鳞癌中有26例MKRN1基因mRNA表达下调，占52％（26／50）；23例HSP90基因mRNA表达上调．占46％（23／50）。MKRN1基因在肿瘤组织中的表达水平为0．74±0.63，在癌旁正常对照组织中为1．21±0．76，（P〈0．01）。HSP90基因在肿瘤组织中表达水平为1．82±1.25．而在癌旁正常对照组织中为1．23±1．04，（P〈0．05），差异具有统计学意义。MKRN1与HSP90基因的表达存在负相关（r=-0．458，P〈0．05）。HSP90基因表达情况与肿瘤的病理分期和患者年龄相关（P〈0．05），MKRN1的表达与肿瘤的临床特征均无相关性（P〉0．05）。结论喉癌组织中MKRN1和HSP90基因mRNA的表达异常存在相关性，二者表达平衡的破坏可能在喉癌的发生发展中起着重要的作用。Objective The activity of telomerase and the length of telomere is decided by the balance between expressions of MKRN1 and HSP90 in normal cells. Disturbance of this balance may lead to carcinogenesis. To investigate the relationship between the expressions of these two genes and the development of laryngeal squamous cell carcinoma（LSCC） ,MKRN1 and HSP90 mRNA levels were detected in 50 cases of LSCC tissues and adjacent normal tissues. Methods RT-PCR was used to detect the mRNA level of MKRN1 and HSP90 in 50 cases of LSCC tissues and adjacent normal tissues. Results The down-regulated MKRN1 expression was found in 26 tumor tissues （52%） and HSP90 overexpression was found in 23 tumor tissues （46%）. Statistic analysis showed MKRN1 expression in LSCC tissues （ 0.74 ± 0.63 ） was lower than that in adjacent normal tissues （ 1.21 ± 0.76） （ P 〈 0.01 ） , and HSP90 expression in tumor tissues （ 1.82 ± 1.25 ） was higher than the adjacent normal tissues （1.23 ±1.04） （P 〈0.05 ）. There was significant inverse correlation between expressions of MKRN1 and HSP90. Overexpression of HSP90 was associated with differentiation degree of LSCC and age of patients （ P 〈0.05 ） . MKRN1 expression had no correlation with clinical feature of LSCC （ P 〉 0.05）. Conclusion Disruption of the balance between MKRN1 and HSP90 expressions may be one of the mechanisms involved in the tumorigenesis of LSCC.

关 键 词：喉鳞状细胞癌 MKRN1 HSP90 

分 类 号：R739.65[医药卫生—肿瘤]