siRNA特异性“沉默”Bim基因对胃腺癌细胞凋亡的影响  

Effects of reducing Bim expression in apoptosis of gastric adenocarcinoma cells via siRNA technology

作　　者：江蓓蕾[1] 叶艳[1] 程文晋[1] 陈思[1] 吴萍[1] 张旭东[1] 张林杰[1] 

机构地区：[1]安徽医科大学免疫学教研室,合肥230032

出　　处：《安徽医科大学学报》2007年第6期595-598,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30572118);安徽省自然科学基金(编号:070413077)

摘　　要：目的研究BH3-only蛋白Bim在紫杉醇诱导胃腺癌细胞凋亡中的作用。方法流式细胞仪PI单染法检测细胞凋亡率;JC-1染色检测线粒体膜电位的变化;在紫杉醇相对敏感SGC-7901胃腺癌细胞株中采用小干扰RNA(siRNA)技术特异性"沉默"Bim基因;Western blot分别检测紫杉醇诱导siRNA"沉默"Bim基因前后Bim蛋白的表达水平变化。结果紫杉醇诱导胃腺癌细胞凋亡具有剂量和时间依赖性,SGC-7901较BGC-823胃腺癌细胞株对紫杉醇更为敏感。紫杉醇诱导SGC-7901及BGC-823胃腺癌细胞很大程度依赖线粒体途径凋亡。特异性"沉默"Bim基因的转录,紫杉醇诱导凋亡敏感细胞株SGC-7901的凋亡率明显降低。结论Bim在紫杉醇诱导胃腺癌细胞凋亡中发挥关键调节作用。Objective To study the role gastric adenocarcinoma cells. Methods of BH3-only proapoptosis protein Apoptosis was measured by flow Bim in apoptosis of paclitaxel-induced cytometry using propidium iodide staining. Changes in mitochondrial membrane potential were studied using JC-1 staining. Bim-specific siRNA and con- trol siRNA was transfected into the sensitive SGC-7901 cells, respectively. Western blot was used to detect the target gene expression at protein levels. Results Paclitaxel induced apoptosis of gastric adenocarcinoma cells in a dose- and time-dependent manner. It appeared that SGC-7901 cells were more sensitive to paclitaxel-induced apoptosis than BGC-823 cells. Paclitaxel induced mitochondrial-dependent apoptosis in SGC-7901 and BGC-823. Inhib- iting Bim expression by siRNA protects gastric adenocarcinoma cells from paclitaxel-induced apoptosis. Conclusion BH3-only protein Bim plays a critical role in paclitaxel-induced apoptosis of gastric adenocarcinoma cells.

关 键 词：胃肿瘤 细胞凋亡 紫杉醇 SIRNA RNA 小分子干扰 

分 类 号：R735.2[医药卫生—肿瘤] R329.2[医药卫生—临床医学]