特布他林对A549细胞吞噬凋亡嗜酸性粒细胞及分泌IL-6和IL-8的影响  

Effects of terbutaline on the engulfing apoptotic eosinophils the secreting IL-6 and IL-8 of A549 cells

作　　者：高坤[1] 汪诚[1] 刘荣玉[1] 

机构地区：[1]安徽医科大学第一附属医院干部呼吸内科,合肥230022

出　　处：《安徽医科大学学报》2007年第6期657-660,共4页Acta Universitatis Medicinalis Anhui

基　　金：科技部973项目(编号:2001CB510009)

摘　　要：目的探讨特布他林对A549细胞吞噬凋亡嗜酸性粒细胞及对A549细胞分泌白介素(IL)-6、IL-8的影响。方法①用磁体活化的细胞分离柱分离嗜酸性粒细胞,培养使其老化凋亡;凋亡嗜酸性粒细胞与不同作用条件的特布他林干预的A549细胞共培养,光学显微镜下观察A549细胞吞噬凋亡嗜酸性粒细胞能力的改变。②对A549细胞给予不同浓度的特布他林干预,采用放射免疫检测IL-6、IL-8浓度。结果①特布他林以时间和浓度依赖的方式抑制A549细胞吞噬凋亡嗜酸性粒细胞。②特布他林抑制脂多糖诱导的A549细胞分泌IL-6、IL-8且存在浓度依赖性。结论特布他林(1、10μmol/L)抑制A549细胞吞噬凋亡嗜酸性粒细胞,低浓度特布他林(0.1μmol/L)无抑制作用,但不同浓度的特布他林均可以通过抑制脂多糖诱导的A549细胞对IL-6、IL-8的分泌起到一定的抗炎作用。Objective To investigate the effects of terbutaline on human apoptotic eosinophils engulfed by A549 cells and secretions of IL-6 and IL-8 from A549 cells. Methods Eosinphils were separated by magneticactivated separation column and cultured for 48 hours to achieve apoptosis, then they were co-cultured with A549 cells treated by terbutaline at the different conditions. Lightmicroscopy was used to observe the engulfing apoptotic eosinophils of A549 cells. The supernatants of cultured A549 cells treated with different doses of terbutaline were collected to measure the concentrations of IL-6 and IL-8 by using radio immunoassay （RIA）. Results Terbutaline inhibited A549 cells from phagocytosing apoptotic eosinophils in time and concentration dependent manners and significantly decreased LPS-induced secretion of IL-6 and IL-8 from A549 cells in concentration-dependent manner. Conclusion Terbutaline （1.10 μmol/L）inhibits A549 cells from engulfing apoptotic eosinophils, but terbutaline （0. 1 μmol/ L） has anti-inflammatory propertie by inhibiting LPS-induced IL-6 and IL-8 secretions from A549 cells.

关 键 词：特布他林/药理学 嗜酸性粒细胞 细胞系 肿瘤 白细胞介素-6 白细胞介素-8 

分 类 号：R734.2[医药卫生—肿瘤] R562.25[医药卫生—临床医学]