PSA及NCAM表达差异COS-7细胞的建立及PSA对COS-7细胞迁移侵袭能力的影响  

Construction of PSA and NCAM differently expressing COS-7 cell line and the effects of PSA on migration and invasion of the diverse COS-7 cell

作　　者：程亚彬[1] 李静[1] 戴功[1] 耿美玉[1] 

机构地区：[1]中国海洋大学医药学院分子药理室,山东青岛266003

出　　处：《中国药理学通报》2007年第12期1565-1570,共6页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(No2003CB716400)

摘　　要：目的构建PSA与NCAM表达差异的COS-7细胞,并检测PSA的表达对细胞粘附、迁移侵袭能力的影响,为今后进一步研究PSA介导的细胞迁移侵袭信号通路变化,阐明PSA对肿瘤转移影响的分子机制奠定基础。方法该文通过双转染质粒构建PSA与NCAM表达差异的COS-7细胞株,利用流式细胞术及Western blot方法检验转染效率;采用MTT法检测PSA表达差异COS-7细胞的增殖;粘附分析法检测COS-7细胞对细胞外基质Fn的粘附能力;利用tran-swell小室比较PSA与NCAM表达差异的COS-7细胞迁移、侵袭能力。结果PSA与NCAM表达差异COS-7细胞构建成功,PSA可降低COS-7细胞与基底膜的粘附,增强其迁移及侵袭能力。结论该文所建立的PSA与NCAM表达差异的COS-7细胞株可用于今后进行PSA对细胞迁移侵袭信号通路影响的机制研究。Aim To construct a polysialic acid （PSA） and neural cell adhesion molecule （NCAM）differently expressing COS-7 cell line, and investigate the effects of PSA on the cell adhesion, migration and invasion, aiming to establish the base for further investigation of the signaling passway of the diverse celluar migration and invasion, and elucidate the molecular mechanism of PSA promoting cancer cell metastasis. Methods A polysialic acid and neural cell adhesion molecule differently expressing COS-7 cell line was constructed by transient cotransfection, and the cotransfection efficiency was determined by Western blot and flow cytometry. Adhesion assay was used to investigate the effect of PSA on cell adhesion ability; transwell assay was used to measure migration and invasion ability. Results The PSA and NCAM differently expressing COS-7 cell line was successfully constructed, which demonstrated PSA inhibited the cell adhesion to basement membrane, and promoted the migration and invasion ability. Conclusions The constructed polysialic acid and neural cell adhesion molecule differently expressing COS-7 cell line can be used to investigate molecular mechanism of promoting cancer cell metastasis induced by PSA in the future.

关 键 词：PSA NCAM COS-7 迁移 侵袭 肿瘤 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R394.2[医药卫生—基础医学]