金钱松内生真菌JJ18杀螺作用实验研究  

Experimental research on molluscicidal effect of endophyte JJ18 from Pseudolarix amabilis

作　　者：郭尚彬[1] 陈钧[1] 王妍[1] 承曦[1] 祁红兵[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013

出　　处：《中国中药杂志》2008年第4期389-392,共4页China Journal of Chinese Materia Medica

基　　金：江苏省2004年度研究生创新计划项目(xm04-57)

摘　　要：目的:研究金钱松内生真菌JJ18的杀螺作用、活性部位及该菌株杀螺活性的传代稳定性,从而探索其应用的可能性。方法:按照WHO的杀螺剂浸泡试验法观察不同处理下JJ18发酵液杀螺效果。结果:JJ18菌株发酵液的活性成分集中在胞外液,当胞外液浓度10%时,72h杀螺率接近90%,且发酵外液成盐后有良好的耐热性,强光照下7 d内活性物质基本不改变,菌株传代稳定,杀螺活性成分集中在正丁醇萃取部位。结论:金钱松内生真菌JJ18代谢产物的杀螺活性,有开发应用的可能性。Objective：To research molluscicidal effect activity,active components and stable passage of endophyte JJ18 from Pseudolarix amabilis and examine the possibility for practical application, nethod：Molluscicidal effect test was performed according to the immersion test method suggested by WHO. Result： Immersion test with JJ18 broth showed that the active components were extracel- lular moiety of the broth and that 10% concentration solution could kill nearly 90% snail immersed after 72 h, the salified broth has favourable thermostabily and photostability and showed that JJ18 has stable passage and its active components concentrate in the extract of n-butanol. Conclusion： The metabolite of endophyte JJ18 has activity for molluscicidal effect and potential for application.

关 键 词：钉螺 内生真菌 杀螺作用 金钱松 JJ18 

分 类 号：R285.5[医药卫生—中药学]