IL-6体外对PC12细胞株DA和NE代谢抑制的机制  

In vitro inhibitory effect of interleukin-6 on the metabolism of DA and NE in PC12 cell line and the related signal transduction

作　　者：李荣[1] 王杰军[1] 沈毅珺 吴欣[1] 王妙苗[1] 王喜[1] 何晓静[1] 

机构地区：[1]中国人民解放军第二军医大学长征医院肿瘤科,上海市200070 [2]上海复旦张江生物医药股份有限公司基因工程研发部,上海市200070

出　　处：《世界华人消化杂志》2008年第1期45-49,共5页World Chinese Journal of Digestology

摘　　要：目的:体外研究IL-6与PC12细胞株神经递质DA,NE合成代谢之间的关系及JAK-STATs途径在其中的作用.方法:用不同剂量的IL-6(0.5 mg/L,1 mg/L和1.5 mg/L)处理分化的PC12细胞.采用磷脂酰丝氨酸外翻法检测细胞凋亡率,应用HPLC检测上清和细胞裂解液中DA和NE的含量.采用1 mg/L IL-6处理分化的PC12细胞,用免疫印迹法(Western blotting)检测IL-6处理不同时间内全细胞裂解液中STAT3、TH蛋白表达水平,浆蛋白及核蛋白裂解液内STAT3和p-sTAT3表达水平.结果:各组细胞凋亡率无明显差别.加入IL-6后,随着浓度的增加,DA和NE代谢呈近似剂量依赖性降低,当IL-6为1 mg/L时DA和NE的变化有统计学意义.1 mg/L IL-6可以增加STAT3蛋白的表达、p-STAT3蛋白的转核、降低酪氨酸强化酶的表达.结论:IL-6通过JAK-STAT3信号转导途径,影响酪氨酸羟化酶的表达,从而降低DA和NE的合成.AIM： To examine the relationship between interleukin （IL）-6 and the anabolism of DA and NE in the PC12 cell line, and to explore the related signal transduction. METHODS： Differentiated PC12 cells were used to examine the inhibitory effect of IL-6 （0.5, 1 and 1.5 mg/L） on the anabolism of DA and NE. Apoptosis of PC12 cells was measured by fluocytometry. The concentration of DA and NE in PC12 cells and the medium was determined by HPLC. After exposure to IL-6 （1 mg/L）, protein expression of p-STAT3 in the nuclei and STAT3 and tyrosine hydroxylase （TH） in the cytoplasm of PC12 cells was measured by Western blotting. RESULTS： The apoptosis rate of PC12 cells cultured in the presence of different concentrations of IL-6 did not differ significantly. The metabolism of DA and NE in PC12 cells changed in accordance with the concentration of IL-6 added to the medium, and it obviously differed from that of the controls at a concentration of 1 mg/L. IL-6 enhanced the protein expression of STAT3 and the transcription to nuclei and decreased protein expression of TH. CONCLUSION： IL-6 reduces the synthesis of DA and NE in vitro. The mechanism underlying the effects of IL-6 on the synthesis of DA and NE involves JAK-STATs signal transduction and decreases protein expression of TH.

关 键 词：信号转导 JAK-STAT3 多巴胺 酪氨酸羟化酶 

分 类 号：R749.4[医药卫生—神经病学与精神病学]