亚麻籽饼粕中开环异落叶松树脂酚二葡萄糖苷的提取工艺研究  

Study on the Extraction Technique of Secoisolariciresinol Diglucoside in the Flaxseed Cake

作　　者：陈志强[1] 高岚[1] 王昌禄[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院天津市食品营养与安全重点实验室

出　　处：《安徽农业科学》2008年第3期855-856,共2页Journal of Anhui Agricultural Sciences

摘　　要：建立了定性分析亚麻籽饼粕中开环异落叶松树脂酚二葡萄糖苷(SDG)的TLC方法,根据TLC结果,确定提取溶剂为0.1 mol KOH溶于1 L 70%乙醇,固液比为1∶8,提取2次。然后用大孔吸附树脂与TLC相结合的方法,探讨了亚麻籽中SDG的分离纯化,并根据HPLC分析结果确定了最佳的分离纯化工艺条件。即当亚麻籽饼粕KOH-乙醇-水提取液经中和浓缩后,用DM-21型大孔吸附树脂吸附,水洗去杂质,60%乙醇洗脱,浓缩干燥,经HPLC检测,亚麻籽饼粕含SDG为1.64%,粗提物产率达4.98%,SDG质量分数为32.93%。结果表明:KOH-乙醇-水提取与大孔吸附树脂相结合制备SDG提取物的方法与传统分离法相比,得率高、工艺操作简单且安全无毒。The lignan secoisolariciresinol diglucoside （SDG） is a bioactive molecule effective on anticancer, antiviras and antioxidation. A simple and direct method for SDG qualitative analysis was established with TLC. Suitable macroporous adsorption resin （MAR DM-21 ） was chosen to separate SDG from extract solvent system （KOH-ethanol-water） of flaxseed cake. The identification of SDG and the determination of the purity of targeted fraction were carried out with HPLC. It was found that DM-21 showed an extract rate as 4.98 % and the purified fraction contained 32.93 % （W/W） SDG. Compared to the conventional extraction method,the yield of SDG was improved significantly and the extract time was greatly reduced. It was considered as a safe and suitable medium for sealed-up purification of SDG.

关 键 词：亚麻籽饼粕 亚麻木酚素 TLC HPLC 

分 类 号：O658[理学—分析化学]