基于AMEL基因的绵羊性别鉴定技术  

Sex Identification Technique in Sheep Based on AMEL Gene

作　　者：管峰[1] 朱嵊[1] 刘军[1] 张明洲[1] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018

出　　处：《安徽农业科学》2008年第3期906-907,共2页Journal of Anhui Agricultural Sciences

基　　金：浙江省自然科学基金项目(Y305107);中国计量学院基金项目(XZ0538)

摘　　要：[目的]探讨将牙釉蛋白(AMEL)基因用于绵羊性别鉴定的可行性。[方法]采用酚-氯仿法从湖羊的耳皮肤组织中提取基因组DNA。分别以公羊和母羊的DNA为模板,以AMEL引物和SRY引物进行PCR扩增。[结果]经扩增后母羊得到一条来自X染色体的270bp条带,而公羊则得到270和210 bp的两条带。两种方法对10份已知DNA样品检测结果与实际性别完全一致。PCR产物的电泳条带较为清晰,相同性别电泳条带数完全一致,而不同性别间电泳条带数和片段大小差异显著。AMEL引物扩增雌性个体DNA实际电泳条带数和理论条带数一致,而来自雄性结果却不一致,这可能与非特异性扩增有关。[结论]AMEL基因比SRY基因具有更高的灵敏性,可用于判定XY染色体动物的性别。[Objective] The aim of the research was to discuss the feasibility of applying Amelngenin （AMEL） gene in the sex identification of sheep. [ Method] Conomic DNA was extracted from the ear skin of Flu sheep by using phenol - chloroform method. With DNA in male Flu sheep and female Flu sheep as templates, AMEL primer and SRY premier were used for PCR amplification. [ Result ] Through amplification, one band of 270 bp from X chromesome was obtained from ewe and two bands of 270 bp and 210 bp were obtained from ram. The detection results of 10 shares of known DNA samples by using two methods were completely accorlant with the actual sex. The electrophoresis bands of PCR products were very clear, the electrophoresis band number in the same sex were aecordant and the band number and fragment length had significant difference airing different sexes. The actual electrophoresis band number of DNA in female individuals after amplification with AMEL primer were accordant with the theoretical electrophoresis band number, but the results from male sheep were not aceordant, which might be related with the non-specific amplification. [ Conclusion] AMEL gene had higher sensitivity than SRY gene and could be applied in judging the sex of the animals with XY chromosome.

关 键 词：AMEL基因 SRY基因 绵羊 性别鉴定 

分 类 号：Q754[生物学—分子生物学]