南极冰藻Berkeleya rutilans H-15中清除自由基活性物质的分离纯化及其结构分析  

Purification and molecular configuration primary analysis of free radical scavenging substances in antarctic ice alga Berkeleya rutilans H-15

作　　者：郑洲[1] 缪锦来[1] 陈豪 王守强[1] 

机构地区：[1]国家海洋局海洋生物活性物质国家海洋局重点实验室,山东青岛266061 [2]山东省眼科学重点实验室省部共建国家重点实验室培育基地,山东青岛266071

出　　处：《中国海洋药物》2008年第1期8-12,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金资助项目(40506005);青岛市科技计划资助项目(06-2-2-21-jch)

摘　　要：目的研究和发现极端环境下南极冰藻中清除自由基活性物质。方法以南极冰藻Berkeleya ruti-lans H-15为试验材料,在国内外首先建立起了从南极冰藻中获取清除自由基活性物质的一套完整的方法,包括用甲醇提取活性化合物,用DPPH法和Godin法来追踪和检测活性化合物的分离效果及变化情况,用硅胶柱层析对活性化合物进行初步的分离和纯化,用SephadexLH柱层析来进一步纯化,通过高效液相来进行化合物纯度检测,高纯度化合物用红外光谱和液质联用方法来确定化合物的类型和有关的功能基团。结果在Berkeleya rutilans H-15中获得了一个纯的活性化合物,经红外光谱和液质联用图谱初步进行分子结构分析,推测此化合物属于酚类化合物,并具有很强的清除自由基的活性。结论在南极冰藻中寻找并获得高活性的抗氧化剂是完全可行的。Objective To study on quenching free radical substances in antarctic ice alga. Methods Antarctic ice alga Berkeleya rutilans H-15 used as material, complete experiment of preparation free radical scavenging substances was established. All of these include the extraction of active substances by methanol, then pursuing and determination of the separation effect and change of active substances by DPPH and Godin methodl the primary separation and purification by silica gel 60 column chromatogram; the farther purification by Sephadex LH column chromatogram and the purity determination of compound by HPLC. The type of compound and functional group of high pure compound were determined by the IR spectrum, HPLC-MS. Results One pure compound with strong activity was obtained from Berkeleya rutilans H-15, and it was confirmed to belong to phenol derives by analysis of IR spectrum and HPLC-MS. Conclusion It was completely feasible to find and acquire quenching free radical substances in antarctic ice alga, which initiated a new way to obtain natural antioxidant active substances from antarctic microorganism.

关 键 词：南极冰藻 硅藻H-15 清除自由基 活性物质 

分 类 号：TQ460[化学工程—制药化工] Q949.2[生物学—植物学]