原位杂交法检测树舌多糖GF对HepA瘤细胞N-ras、C-myc基因mRNA表达的影响  

Effect of Ganoderma appanatum polysaccharides GF on N-ras and C-myc oncogene expression in mouse HepA tumour

作　　者：宋高臣[1] 于英君[2] 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《成都中医药大学学报》2007年第4期33-34,共2页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：黑龙江省科技攻关项目(项目号:G00C200902)

摘　　要：目的:探明树舌多糖GF对HepA瘤细胞癌基因N-ras、C-myc mRNA水平的影响。方法:应用地高辛标记探针原位杂交技术,检测树舌多糖GF对HepA瘤细胞中癌基因N-ras、C-myc mRNA丰度的影响。结果:树舌多糖组、猪苓多糖组中mRNA表达量均显著低于模型组(P(0.01),树舌多糖组与猪苓多糖组无显著性差异。结论:树舌多糖GF可通过调节N-ras、C-myc基因mRNA的表达,而抑制HepA瘤细胞的增殖。Objective： To study the effect of GAPSGF on N -ras , C -myc mRNA expression in HepA turnout. Methods . ISH were used to determine the effect of Ganoderma appanatum polysaccharides GF on N - ras , C - myc mRNA in mouse HepA tumor. Reaults： The average levels of N - ras , C - myc expression in GAPSGF group and PUPS group have much difference with control group. That is to say, GAPSGF and PUPS can significantly reduce the N -ras , C -myc mRNA expression in HepA cells. Conclusion： Regulating the N -ras , C -myc oncogene mRNA expression is the mechanisms of antitumor activity.

关 键 词：树舌多糖GF HEPA瘤细胞 N-RAS C-MYC 原位杂交 

分 类 号：R284[医药卫生—中药学] R73-74[医药卫生—中医学]