睾丸生殖细胞肿瘤中CD117的表达及其对精原细胞瘤的鉴别意义  

Expression of CD117 in Human Testicular Germ Cell Tumors and Its Diagnostic Value for Seminoma and Nonseminoma

作　　者：刘东亮[1] 卢一平[2] 石海燕[1] 朱蜀侠[1] 吕健为[1] 李卫峰[3] 荣霞[4] 

机构地区：[1]中国人民解放军第363医院泌尿外科,四川成都610041 [2]四川大学华西医院泌尿外科,四川成都610041 [3]中国人民解放军第363医院病理科,四川成都610041 [4]中国人民解放军第363医院检验科,四川成都610041

出　　处：《中华男科学杂志》2008年第1期38-41,共4页National Journal of Andrology

摘　　要：目的:探讨CD117在睾丸生殖细胞肿瘤中的表达及其在鉴别睾丸精原细胞瘤和非精原细胞瘤中的价值和生物学意义。方法:采用CD117单克隆抗体对74例睾丸生殖细胞肿瘤和20例正常睾丸组织进行免疫组化染色,测定不同组织中CD117表达的阳性率、染色密度和染色强度,采用免疫反应积分(IRS)对结果进行分析比较。结果:74例睾丸生殖细胞肿瘤中,45例(60.8%)CD117表达阳性,IRS为(3.89±3.41)分。32例精原细胞瘤中,CD117阳性表达31例,阳性率为96.9%,IRS(6.82±2.76)分,表达部位以细胞膜为主;11例混合性精原细胞瘤中,CD117阳性表达10例,阳性率为90.9%,均为在精原细胞瘤成分中呈弱阳性表达,IRS为(1.25±0.42)分;31例非精原细胞瘤中CD117表达阳性者仅4例,阳性率为12.9%,并且均为胞质内弱阳性染色,IRS仅为(0.60±0.16)分。不同组织来源的睾丸生殖细胞肿瘤两两之间CD117表达差异均有统计学意义(P<0.05)。20例正常睾丸组织CD117均为阳性表达,IRS为(7.30±1.89)分。结论:CD117在睾丸精原细胞瘤细胞膜上有较为特异的表达,其在睾丸生殖细胞肿瘤中的表达对于鉴别精原细胞瘤和非精原细胞瘤有重要价值。Objective： To evaluate the expression of CD117 in human testicular germ cell tumors and its value in the differential diag- nosis of seminoma and nonseminoma. Methods： Seventy-four human testicular germ cell tumor specimens were studied by ABC kit immunohistochemical staining detection using CD117 monoclonal antibodies. The immunoreaction scores（ILLS） of all the specimens were calculated and analysed for their clinical significance. Results ： Among the 74 germ cell tumors, 31 out of 32 （9 6.9% ） semi-nomas showed positive staining of the CD117 mostly on the cell membrane. Four of 31 （ 12.9% ） nonseminomas displayed a weak posi-tive staining of CD117 only in the cytoplasm of a few cells. In 10 of 11 mixed germ cell tumors, a relatively weak expression of CD117 was shown only in the seminoma component. The CDll7 expression was diagnostically decreased from seminoma to mixed seminoma and to nonseminoma successively, with IRS of 6.82 ~ 2.76, 1.25 ~ 0.42 and 0.60 ~ 0.16, respectively. There was a significant difference in the CD117 expression between seminoma and nonseminoma （ P 〈 0.05）. CD117 proteins were positively expressed in all the 20 specimens of the normal testis. Conclusion： The detection of CD117 by immunohistochemical staining using CD117 mono- clonal antibodies is a newly developed useful method for differentiating seminoma and nonseminoma.

关 键 词：CD117 睾丸生殖细胞肿瘤 精原细胞瘤 非精原细胞瘤 免疫组织化学 

分 类 号：R737.21[医药卫生—肿瘤]